mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense

• Published in: World journal of gastroenterology : WJG 2004-05, Vol.10 (10), p.1528-1532
• Main Authors: Yuan, Jian-Ping, Li, Tao, Li, Zhen-Hong, Yang, Gui-Zhen, Hu, Bao-Yu, Shi, Xiao-Dong, Shi, Tie-Liu, Tong, Shan-Qing, Guo, Xiao-Kui
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacterial Toxins - genetics; Bacterial Toxins - immunology; Brief Reports; Cell Line; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Helicobacter pylori - genetics; Helicobacter pylori - metabolism; Humans; Immune System - physiology; mRNA表达; Oligonucleotide Array Sequence Analysis; RNA, Messenger - genetics; RNA, Messenger - metabolism; 免疫逃逸; 幽门螺杆菌; 消化道溃疡; 细胞毒素
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i10.1528

AIM: To study the immune response of host to Helicobacter pylori VacA.METHODS: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h postinfection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot.RESULTS: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion.CONCLUSION: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.