Expression of MTLC gene in gastric carcinoma

To investigate the expression of c-myc target from laryngeal cancer cells (MTLC) gene in gastric carcinoma (GC) tissues and the effect of MTLC over-expression on gastric carcinoma cell line BGC823. RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues. BGC82...

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Published in:World journal of gastroenterology : WJG 2003-10, Vol.9 (10), p.2160-2163
Main Authors: Qiu, Guang-Bin, Gong, Li-Guo, Hao, Dong-Mei, Zhen, Zhi-Hong, Sun, Kai-Lai
Format: Article
Language:English
Subjects:
Apoptosis - physiology
Cell Division - physiology
Cell Line, Tumor
Down-Regulation - physiology
Gastric Cancer
Gene Expression Regulation, Neoplastic
Humans
Nuclear Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms - pathology
Stomach Neoplasms - physiopathology
Transfection
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cited_by cdi_FETCH-LOGICAL-c418t-c0dcaa3aa30ec1b2158207ef5a16d1f7c006d28f7e6f8c4c96470e3fb56535233
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container_issue 10
container_start_page 2160
container_title World journal of gastroenterology : WJG
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creator Qiu, Guang-Bin
Gong, Li-Guo
Hao, Dong-Mei
Zhen, Zhi-Hong
Sun, Kai-Lai
description To investigate the expression of c-myc target from laryngeal cancer cells (MTLC) gene in gastric carcinoma (GC) tissues and the effect of MTLC over-expression on gastric carcinoma cell line BGC823. RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues. BGC823 cells were transfected with an expression vector pcDNA3.1-MTLC by liposome and screened by G418. Growth of cells expressing MTLC was observed daily by manual counting. Apoptotic cells were determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay. The expression of MTLC mRNAs was down-regulated in 9(60%) of 15 cases of GC tissues. The growth rates of the BGC823 cells expressing MTLC were indistinguishable from that of control cells. A marked acceleration of apoptosis was observed in MTLC-expressing cells. MTLC was down-regulated in the majority of GC tissues and could promote apoptosis of GC cell lines, which suggests that MTLC may play an important role in the carcinogenesis of gastric carcinoma.
doi_str_mv 10.3748/wjg.v9.i10.2160
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RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues. BGC823 cells were transfected with an expression vector pcDNA3.1-MTLC by liposome and screened by G418. Growth of cells expressing MTLC was observed daily by manual counting. Apoptotic cells were determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay. The expression of MTLC mRNAs was down-regulated in 9(60%) of 15 cases of GC tissues. The growth rates of the BGC823 cells expressing MTLC were indistinguishable from that of control cells. A marked acceleration of apoptosis was observed in MTLC-expressing cells. 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subjects Apoptosis - physiology
Cell Division - physiology
Cell Line, Tumor
Down-Regulation - physiology
Gastric Cancer
Gene Expression Regulation, Neoplastic
Humans
Nuclear Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms - pathology
Stomach Neoplasms - physiopathology
Transfection
title Expression of MTLC gene in gastric carcinoma
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