Effects of Rheum tanguticum polysaccharide on TNBS -induced colitis and CD4+T cells in rats

• Published in: World journal of gastroenterology : WJG 2003-10, Vol.9 (10), p.2284-2288
• Main Authors: Liu, Li, Wang, Zhi-Peng, Xu, Chang-Tai, Pan, Bo-Rong, Mei, Qi-Bing, Long, Yin, Liu, Jia-Yun, Zhou, Si-Yuan
• Format: Article
• Language: English
• Subjects: Animals; Basic Research; Body Weight - drug effects; CD4-Positive T-Lymphocytes - drug effects; Colitis - chemically induced; Colitis - drug therapy; Colitis - immunology; Drugs, Chinese Herbal; Intestinal Mucosa - metabolism; Male; Organ Size - drug effects; Peroxidase - metabolism; Phytotherapy; Polysaccharides - pharmacology; Rats; Rats, Sprague-Dawley; Rheum; Spleen - anatomy & histology; Superoxide Dismutase - metabolism; Thymus Gland - anatomy & histology; Trinitrobenzenesulfonic Acid
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i10.2284

To study the effects of Rheum tanguticum polysaccharide(-1) (RTP(-1)) on ulcerative colitis in rats induced by 2, 4, 6-trinitrophene sulphonic acid (TNBS) and their possible mechanism. RTP1 (200 mg.kg(-1), i.g.) extracted from Rheum tanguticum Maxim. ex Regel was administrated to rats with colitis induced by TNBS for 5 d, 7 d, 10 d and 14 d, respectively. The effects of RTP1 and dexamethasone (DX, 0.2 mg.kg(-1), i.g.) were contrastively investigated. The MPO level and SOD activity were determined by chromatometry. The expansion and protein expression of CD4+T lymphocytes isolated from colon mucosae and mesenteric lymph nodes of colitis rats were performed by immunohistochemical analysis and Western-blot methods. Treatments of RTP1 (200 mg.kg(-1), i.g.) significantly reduced diarrhea, mortality, colon mass, ulcer areas and MPO level in colon mucosae on days 5, 7, 10 and 14 (5.2+/-1.4, 5.4+/-0.7, 5.2+/-1.8, P