Screening and identification of proteins mediating senna induced gastrointestinal motility enhancement in mouse colon

To isolate the proteins involved in pharmacologic action of senna extract (SE) from mouse gastrointestinal tract and to explore the molecular mechanism of gastrointestinal motility change induced by SE. SE was administrated to mice by different routes. Gastrointestinal motility of mice was observed...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2002-02, Vol.8 (1), p.162-167
Main Authors: Wang, Xin, Zhong, Yue-Xia, Lan, Mei, Zhang, Zong-You, Shi, Yong-Quan, Lu, Ju, Ding, Jie, Wu, Kai-Cun, Jin, Jian-Ping, Pan, Bo-Rong, Fan, Dai-Min
Format: Article
Language:English
Subjects:
Animals
Basic Research
Cathartics - pharmacology
Colon - chemistry
Colon - drug effects
Colon - physiology
Diarrhea - chemically induced
Diarrhea - diagnostic imaging
Diarrhea - physiopathology
Disease Models, Animal
Electrophoresis, Gel, Two-Dimensional
Enteral Nutrition
Female
Gastrointestinal Motility - drug effects
Injections, Subcutaneous
Intestinal Mucosa - chemistry
Intestinal Mucosa - drug effects
Intestinal Mucosa - ultrastructure
Isoelectric Focusing
Male
Mice
Mice, Inbred BALB C
Plant Extracts - pharmacology
Proteins - analysis
Radiography
Senna Plant
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Summary:To isolate the proteins involved in pharmacologic action of senna extract (SE) from mouse gastrointestinal tract and to explore the molecular mechanism of gastrointestinal motility change induced by SE. SE was administrated to mice by different routes. Gastrointestinal motility of mice was observed using cathartic, gastrointestinal propellant movement experiments and X-ray analysis. Mouse model for gastrointestinal motility enhancement was established through continuous gastric administration of SE at progressively increased dose. At 3 h and week 3, 4, 6 and 10, morphological changes of gastrointestinal tissues were found under light microscope. Ultrastructural changes of intestinal and colonic tissues at week 6 were observed under transmission electron microscope. The colonic proteomic changes in model mice were examined by two-dimension polyacrylamide gel electrophoresis with immobilized pH gradient isoelectric focusing to screen the differentially expressed proteins, and their molecular masses and isoelectric points were determined. Two N-terminal sequences of the samples were also determined by mass spectrometry. SE (0.3g) caused diarrhea after gastric administration in 1-6h and enhanced gastrointestinal propellant (65.1+/-7.5%; 45.8+/-14.6%, P
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v8.i1.162