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The abnormal expression of retinoic acid receptor—β,p53 and Ki67 protein in normal,premalignant and malignant esophageal tissues
AIM: Esophageal cancer remains a significant health problem worldwide. It is important to investigate alterations in expression of retinoic acid receptor-β, P 53 and Ki67 proteins in esophageal carcinogenesis. METHODS: To find biomarkers for early identification of esophageal cancer, we analyzed the...
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Published in: | World journal of gastroenterology : WJG 2002-04, Vol.8 (2), p.200-202 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | AIM: Esophageal cancer remains a significant health problem worldwide. It is important to investigate alterations in expression of retinoic acid receptor-β,
P
53 and Ki67 proteins in esophageal carcinogenesis.
METHODS: To find biomarkers for early identification of esophageal cancer, we analyzed the retinoic acid receptor-β,
P
53 protein and the proliferation marker Ki67 in surgical specimens of normal, mildly, and severely dysplastic and malignant esophageal tissues by
in situ
hybridization of RNA and immunohistochemistry.
RESULTS: RAR-β was expressed in 94.3% (33/35) of normal mucosae, 67.8% (19/28) of the mild, 58.1% (18/31) of the severe lesions and 53.2% (116/218) of tumor samples. RAR-β mRNA was expressed in 62.7% (42/67), 55.1% (43/78) and 29.2% (7/24) of well, moderated and poorly differentiated SSCs. The
P
53 and Ki67 proteins were 5.9% (2/34) of the normal mucosa. P53 and Ki67 stained positively in 10.7% (3/28) and 21.4% (6/28) of mild dysplasia, and 51.6% (16/31) and 58.1% (18/31) of severely dysplasia respectively. Samples from esophageal cancer showed no higher levers of
P
53 and Ki67 expression than seen in severely dysplastic lesions. There was significant difference of RAR-β、
P
53 and Ki67 expression between normal mucosa and dysplatic tissue or esophageal cancer.
CONCLUSION: Loss of RAR-β expression and accumulation of
P
53 and Ki67 proteins may serve as biomarkers for early identification of esophageal cancer in the high-risk populations. |
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ISSN: | 1007-9327 2219-2840 |
DOI: | 10.3748/wjg.v8.i2.200 |