Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbits

• Published in: International journal of clinical and experimental medicine 2015-01, Vol.8 (9), p.15692-15699
• Main Authors: Wang, Xiu-Hong, Zeng, Jian-Feng, Lin, Chao, Chen, Shi-Biao
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 1940-5901; 1940-5901

This study aims to explore the treatment method of myocardial ischemia-reperfusion injury. Myocardial Ischemia-reperfusion rabbit model was established in this study. They were divided into four groups: sham operation (S) group, IRI control (I/R) group and IRI with morphine (MF) group and sufentanil (SF). Myocardial infarct size was compared with HE staining method. TUNEL assay was used to detect cell apoptosis. Myocardial infarct size of control group and morphine and sufetanil group was 36.0±3.6, 23.0±1.2 and 27.1±2.3, respectively. There were significant differences between them (P < 0.01). Apoptotic index of I/R, MF and SF groups was 26.9±2.2, 12.5±2.3, 15.8±2.0, with statistical significance (P < 0.05). The concentration of CK-MB in serum: there were no significant differences of CK-MB between each group at baseline. The concentration of CK-MB after reperfusion were higher than that of baseline, except for group S (P < 0.05); Compared with group S, after reperfusion, the CK-MB of other three groups were higher (P < 0.05); The concentration of CK-MB in group MF and SF were lower than group I/R (P < 0.05); In contrast to group MF, the concentration of CK-MB after reperfusion was higher in group SF (P < 0.05). Morphine and sufentanil can specifically protect the myocardial function.