Yersinia pseudotuberculosis IP32953 survives and replicates in trophozoites and persists in cysts of Acanthamoeba castellanii

Yersinia pseudotuberculosis is a foodborne enteric pathogen that causes a mild self-limiting diarrhea in humans. Yersinia pseudotuberculosis is able to persist in soil and water and in association with fresh produce, but the mechanism by which it persists is unknown. It has been shown that Y. pseudo...

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Bibliographic Details
Published in:FEMS microbiology letters 2015-07, Vol.362 (13), p.fnv091-fnv091
Main Authors: Santos-Montañez, Jennifer, Benavides-Montaño, Javier A., Hinz, Angela K., Vadyvaloo, Viveka
Format: Article
Language:English
Subjects:
Acanthamoeba castellanii
Acanthamoeba castellanii - microbiology
Acanthamoeba castellanii - physiology
Amoeba
Animals
Coculture Techniques
Cysts
Cytotoxicity
Diarrhea
Food Microbiology
Food Safety
Humans
Life Cycle Stages
Microbial Viability
Microbiology
Pseudotuberculosis
Research Letter
Soil water
Survival
Trophozoites
Trophozoites - microbiology
Vacuoles
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis - growth & development
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Summary:Yersinia pseudotuberculosis is a foodborne enteric pathogen that causes a mild self-limiting diarrhea in humans. Yersinia pseudotuberculosis is able to persist in soil and water and in association with fresh produce, but the mechanism by which it persists is unknown. It has been shown that Y. pseudotuberculosis co-occurs with protozoans in these environments; therefore, this study investigates if bacterivorous free-living amoeba (FLA) are able to support persistence of Y. pseudotuberculosis. Coculture studies of Y. pseudotuberculosis and the prototype FLA, Acanthamoeba castellanii revealed that bacteria had an enhanced capacity to survive in association with amoeba and in the absence of any cytotoxic effects. Yersinia pseudotuberculosis is able to survive and replicate in trophozoites specifically localized within vacuoles, and persists within cysts over a period of at least a week. These data present the first evidence that Y. pseudotuberculosis is able to resist the bacterivorous nature of FLA and instead exhibits an enhanced ability to replicate and persist in coculture with amoeba. This study sheds light on the potential role of FLA in the ecology of Y. pseudotuberculosis which may have implications for food safety. Yersinia pseudotuberculosis–amoeba interactions.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnv091