The effect of protocol for disinfection of extracted teeth recommended by center for disease control (CDC) on microhardness of enamel and dentin

• Published in: Journal of clinical and experimental dentistry 2015-12, Vol.7 (5), p.e552-e556
• Main Authors: Salem-Milani, Amin, Zand, Vahid, Asghari-Jafarabadi, Mohammad, Zakeri-Milani, Parvin, Banifatemeh, Alireza
• Format: Article
• Language: English
• ISSN: 1989-5488; 1989-5488
• DOI: 10.4317/jced.52280

According to the guideline of the United States center for disease control (CDC), the extracted teeth should be sterilized by autoclaving or storage in 10% formalin before using for educational or research purposes. The objective of this study was to evaluate the effect of this protocol on microhardness of dentin and enamel. Thirty extracted single-root teeth were used in this study. The crowns were resected, and the roots were longitudinally sectioned into two halves. The Vickers microhardness (VHN) of specimens was measured on polished canal dentin and buccal enamel surfaces. The crowns were randomly divided into three groups (n=10). Group 1 and 2 were sterilized using autoclave and formalin, respectively while group 3 (control) was stored in synthetic tissue fluid. The root halves were also randomly divided into 3 groups (n=20) which were treated as mentioned above for crown samples. Following sterilization, VHN of samples was measured again. ANOVA and paired samples t-tests were used to analyze the data. Autoclaving caused a significant reduction in microhardness of dentin (P