miR-23b as a potential tumor suppressor and its regulation by DNA methylation in cervical cancer

The aberrant expression of miR-23b is involved in the development and progression of cancer. The aim of this study was to evaluate the potential role of methylation in the silencing of miR-23b in cervical cancer cell lines and to determine its expression in stages of malignant progression and in cer...

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Bibliographic Details
Published in:Infectious agents and cancer 2015-11, Vol.10 (1), p.42-42, Article 42
Main Authors: Campos-Viguri, Gabriela Elizabeth, Jiménez-Wences, Hilda, Peralta-Zaragoza, Oscar, Torres-Altamirano, Gricenda, Soto-Flores, Diana Guillermina, Hernández-Sotelo, Daniel, Alarcón-Romero, Luz Del Carmen, Jiménez-López, Marco Antonio, Illades-Aguiar, Berenice, Fernández-Tilapa, Gloria
Format: Article
Language:English
Subjects:
Cancer
Cervical cancer
Development and progression
DNA
Genetic aspects
Methylation
Oncology, Experimental
Tumors
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Summary:The aberrant expression of miR-23b is involved in the development and progression of cancer. The aim of this study was to evaluate the potential role of methylation in the silencing of miR-23b in cervical cancer cell lines and to determine its expression in stages of malignant progression and in cervical cancer tissues HPV16-positive. The methylation of the miR-23b promoter was determined in HeLa, SiHa, CaSki and C33A cells using a Human Cancer miRNA EpiTectMethyl II Signature PCR Array®. The cells were treated with 5-Aza-2'-deoxycytidine, and the expression of miR-23b, uPa, c-Met and Zeb1 was determined by qRT-PCR. miR-92a and GAPDH were used as controls. The expression of miR-23b was determined in cervical scrapes and biopsies of women without squamous intraepithelial lesions, with precursor lesions and with cervical cancer, all were HPV16-positive. The Fisher exact and Mann-Whitney tests were used to compare the differences of the expression of miR-23b, uPa, c-Met and Zeb1 among cell groups, and the difference among patients, respectively. The association between the expression of miR-23b and cervical cancer was determined by logistic regression with a confidence level of 95 %. A value of p 
ISSN:1750-9378
1750-9378
DOI:10.1186/s13027-015-0037-6