Effect of gyromagnetic fields on human prostatic adenocarcinoma cells

To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms. PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8...

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Bibliographic Details
Published in:OncoTargets and therapy 2015-01, Vol.8, p.3489-3497
Main Authors: Lei, Hongen, Xu, Yongde, Guan, Ruili, Li, Meng, Hui, Yu, Gao, Zhezhu, Yang, Bicheng, Xin, Zhongcheng
Format: Article
Language:English
Subjects:
Adenocarcinoma
Antigens
Apoptosis
Arthritis
Breast cancer
Cancer therapies
Cell culture
Cell growth
Cellular signal transduction
Development and progression
Electromagnetism
Flow cytometry
Health aspects
Hospitals
Kinases
Magnetic fields
Mortality
Original Research
Prostate cancer
Proteins
Systematic review
Urology
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Summary:To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms. PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3. Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group. GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.
ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S95306