Enhanced virome sequencing using targeted sequence capture

Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral populations. It is culture independent, requires no a priori knowledge of the viruses in the sample, and may provide useful genomic information. However, MSS can lack sensitivity and may yield insufficient data for de...

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Bibliographic Details
Published in:Genome research 2015-12, Vol.25 (12), p.1910-1920
Main Authors: Wylie, Todd N, Wylie, Kristine M, Herter, Brandi N, Storch, Gregory A
Format: Article
Language:English
Subjects:
DNA, Viral
Genome, Viral
High-Throughput Nucleotide Sequencing - methods
Humans
Metagenome
Metagenomics - methods
Method
RNA, Viral
Sensitivity and Specificity
Viruses - classification
Viruses - genetics
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Summary:Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral populations. It is culture independent, requires no a priori knowledge of the viruses in the sample, and may provide useful genomic information. However, MSS can lack sensitivity and may yield insufficient data for detailed analysis. We have created a targeted sequence capture panel, ViroCap, designed to enrich nucleic acid from DNA and RNA viruses from 34 families that infect vertebrate hosts. A computational approach condensed ∼1 billion bp of viral reference sequence into
ISSN:1088-9051
1549-5469
DOI:10.1101/gr.191049.115