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Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities
Depolarization of neurons in 3-week-old rat hippocampal cultures promotes a rapid increase in the density of surface NMDA receptors (NRs), accompanied by transient formation of nonsynaptic NMDA receptor clusters or NR islands. Islands exhibit cytoplasmic dense material resembling that at postsynapti...
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Published in: | eNeuro 2015-11, Vol.2 (6), p.ENEURO.0066-15.2015 |
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description | Depolarization of neurons in 3-week-old rat hippocampal cultures promotes a rapid increase in the density of surface NMDA receptors (NRs), accompanied by transient formation of nonsynaptic NMDA receptor clusters or NR islands. Islands exhibit cytoplasmic dense material resembling that at postsynaptic densities (PSDs), and contain typical PSD components, including MAGUKS (membrane-associated guanylate kinases), GKAP, Shank, Homer, and CaMKII detected by pre-embedding immunogold electron microscopy. In contrast to mature PSDs, islands contain more NMDA than AMPA receptors, and more SAP102 than PSD-95, features that are shared with nascent PSDs in developing synapses. Islands do not appear to be exocytosed or endocytosed directly as preformed packages because neurons lacked intracellular vacuoles containing island-like structures. Islands form and disassemble upon depolarization of neurons on a time scale of 2-3 min, perhaps representing an initial stage in synaptogenesis. |
doi_str_mv | 10.1523/ENEURO.0066-15.2015 |
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Islands exhibit cytoplasmic dense material resembling that at postsynaptic densities (PSDs), and contain typical PSD components, including MAGUKS (membrane-associated guanylate kinases), GKAP, Shank, Homer, and CaMKII detected by pre-embedding immunogold electron microscopy. In contrast to mature PSDs, islands contain more NMDA than AMPA receptors, and more SAP102 than PSD-95, features that are shared with nascent PSDs in developing synapses. Islands do not appear to be exocytosed or endocytosed directly as preformed packages because neurons lacked intracellular vacuoles containing island-like structures. 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Islands form and disassemble upon depolarization of neurons on a time scale of 2-3 min, perhaps representing an initial stage in synaptogenesis.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Guanylate Kinases - analysis</subject><subject>Guanylate Kinases - physiology</subject><subject>Hippocampus - metabolism</subject><subject>Intracellular Signaling Peptides and Proteins - analysis</subject><subject>Intracellular Signaling Peptides and Proteins - physiology</subject><subject>Mice</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurons - metabolism</subject><subject>New Research</subject><subject>Post-Synaptic Density - metabolism</subject><subject>Receptors, AMPA - analysis</subject><subject>Receptors, AMPA - metabolism</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>Synapses - metabolism</subject><issn>2373-2822</issn><issn>2373-2822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpVkd1O3DAQhS3UqiDKE1RCvuxNtraTOM5NJcQuZSUIFSrXln8m1FVip3aCRB-gz12vlq7gytbMOccz_hD6RMmK1qz8suk2D_d3K0I4L2i9YoTWR-iElU1ZMMHYu1f3Y3SW0i9CCOWsoYJ-QMeMc15TXp2gv2uYwqCi-6NmFzwOPb520xSMGic14A6WGHzCW28XAwlfhTgehF3uPHs1zc7g7nZ9ge_BwDSHiLdpUN6mXEgw6sH5R9ypZMDP-HtI88G1Bp_c7CB9RO97NSQ4ezlP0cPV5sfldXFz9217eXFTmKqhcyFAW6HbXveEGEO5sIyWyjZgKQdKdN8A09DWrBVt27KqtaJnVuueC65qTctT9HWfOy16BLubKKpBTtGNKj7LoJx82_Hup3wMT7LiDWOkzgGfXwJi-L1AmuXo8mJD3hfCkiRt6vzRTJAqS8u91MSQUoT-8AwlcgdR7iHKHcRckDuI2XX-esKD5z-y8h8JK5zv</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Tao-Cheng, Jung-Hwa</creator><creator>Azzam, Rita</creator><creator>Crocker, Virginia</creator><creator>Winters, Christine A</creator><creator>Reese, Tom</creator><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5416-1473</orcidid></search><sort><creationdate>20151101</creationdate><title>Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities</title><author>Tao-Cheng, Jung-Hwa ; Azzam, Rita ; Crocker, Virginia ; Winters, Christine A ; Reese, Tom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-8ebd8b9fbf00cc168d213ad7ed16e10bf7e2be95298999249d8f2dbbf686a5b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Guanylate Kinases - analysis</topic><topic>Guanylate Kinases - physiology</topic><topic>Hippocampus - metabolism</topic><topic>Intracellular Signaling Peptides and Proteins - analysis</topic><topic>Intracellular Signaling Peptides and Proteins - physiology</topic><topic>Mice</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurons - metabolism</topic><topic>New Research</topic><topic>Post-Synaptic Density - metabolism</topic><topic>Receptors, AMPA - analysis</topic><topic>Receptors, AMPA - metabolism</topic><topic>Receptors, N-Methyl-D-Aspartate - metabolism</topic><topic>Synapses - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao-Cheng, Jung-Hwa</creatorcontrib><creatorcontrib>Azzam, Rita</creatorcontrib><creatorcontrib>Crocker, Virginia</creatorcontrib><creatorcontrib>Winters, Christine A</creatorcontrib><creatorcontrib>Reese, Tom</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>eNeuro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao-Cheng, Jung-Hwa</au><au>Azzam, Rita</au><au>Crocker, Virginia</au><au>Winters, Christine A</au><au>Reese, Tom</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities</atitle><jtitle>eNeuro</jtitle><addtitle>eNeuro</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>2</volume><issue>6</issue><spage>ENEURO.0066-15.2015</spage><pages>ENEURO.0066-15.2015-</pages><issn>2373-2822</issn><eissn>2373-2822</eissn><abstract>Depolarization of neurons in 3-week-old rat hippocampal cultures promotes a rapid increase in the density of surface NMDA receptors (NRs), accompanied by transient formation of nonsynaptic NMDA receptor clusters or NR islands. Islands exhibit cytoplasmic dense material resembling that at postsynaptic densities (PSDs), and contain typical PSD components, including MAGUKS (membrane-associated guanylate kinases), GKAP, Shank, Homer, and CaMKII detected by pre-embedding immunogold electron microscopy. In contrast to mature PSDs, islands contain more NMDA than AMPA receptors, and more SAP102 than PSD-95, features that are shared with nascent PSDs in developing synapses. Islands do not appear to be exocytosed or endocytosed directly as preformed packages because neurons lacked intracellular vacuoles containing island-like structures. Islands form and disassemble upon depolarization of neurons on a time scale of 2-3 min, perhaps representing an initial stage in synaptogenesis.</abstract><cop>United States</cop><pub>Society for Neuroscience</pub><pmid>26665164</pmid><doi>10.1523/ENEURO.0066-15.2015</doi><orcidid>https://orcid.org/0000-0001-5416-1473</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cells, Cultured Guanylate Kinases - analysis Guanylate Kinases - physiology Hippocampus - metabolism Intracellular Signaling Peptides and Proteins - analysis Intracellular Signaling Peptides and Proteins - physiology Mice Nerve Tissue Proteins - metabolism Neurons - metabolism New Research Post-Synaptic Density - metabolism Receptors, AMPA - analysis Receptors, AMPA - metabolism Receptors, N-Methyl-D-Aspartate - metabolism Synapses - metabolism |
title | Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities |
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