Discriminating Active Tuberculosis from Latent Tuberculosis Infection by flow cytometric measurement of CD161-expressing T cells

• Published in: Scientific reports 2015-12, Vol.5 (1), p.17918-17918, Article 17918
• Main Authors: Yang, Qianting, Xu, Qian, Chen, Qi, Li, Jin, Zhang, Mingxia, Cai, Yi, Liu, Haiying, Zhou, Yiping, Deng, Guofang, Deng, Qunyi, Zhou, Boping, Kornfeld, Hardy, Chen, Xinchun
• Format: Article
• Language: English
• Subjects: 13; 13/31; 631/250/2499; 692/4017; Antigens, Surface - metabolism; Bacteriology; Biomarkers; Cohort Studies; Diagnosis, Differential; Flow Cytometry - methods; Humanities and Social Sciences; Humans; Immunology; Immunophenotyping; Infections; Interferon-gamma Release Tests; Latent Tuberculosis - blood; Latent Tuberculosis - diagnosis; Latent Tuberculosis - immunology; Latent Tuberculosis - metabolism; Latent Tuberculosis - microbiology; Lymphocyte Count; Lymphocytes; Lymphocytes T; Monocytes; multidisciplinary; NK Cell Lectin-Like Receptor Subfamily B - metabolism; Reproducibility of Results; Science; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism; Tuberculosis; Tuberculosis - blood; Tuberculosis - diagnosis; Tuberculosis - immunology; Tuberculosis - metabolism; Tuberculosis - microbiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep17918

Interferon-gamma Release Assays (IGRAs) significantly increases the possibility for early diagnosis of tuberculosis, but IGRAs alone cannot discriminate active TB from LTBI. Therefore, fast and reliable discrimination of active tuberculosis, especially bacteriology negative tuberculosis, from LTBI is a great necessity. Here we established an assay based on flow cytometric multiparameter assay assessing expression of CD161 along with CD3, CD4 and CD8, whereby a set of indices formulated by the percentages of CD3 + CD161 + , CD3 + CD4 + CD161 + and CD3 + CD8 + CD161 + T cells multiplied with lymphocyte/monocyte ratio were established. Application of the CD3 + CD8 + CD161 + index to compare a cohort of active tuberculosis with a cohort of LTBI or health control yielded 0.7662 (95% confidence interval [CI] 0.6559–0.8552) or 0.7922 (95%  CI 0.6846–0.8763) for sensitivity and 0.9048 (95%  CI 0.8209–0.9580) or 0.8939 (95% CI 0.8392–0.9349) for specificity when the TB cohort was AFB + ; the corresponding results were 0.7481 (95%  CI 0.6648–0.8198) or 0.7557 (95%  CI 0.6730–0.8265) for sensitivity and 0.8571 (95%  CI 0.7637–0.9239) or 0.8603 (95%  CI 0.8008–0.9075) for specificity when the TB cohort was AFB − . Our results reveal that in combination with IGRAs, CD161-based indices provide a novel, fast diagnostic solution addressing the limitation of current tuberculosis diagnostics.