Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: Effect on M1 inflammatory markers

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2015-12, Vol.153, p.344-351
• Main Authors: Fernandes, Kristianne Porta Santos, Souza, Nadhia Helena Costa, Mesquita-Ferrari, Raquel Agnelli, Silva, Daniela de Fatima Teixeira da, Rocha, Lilia Alves, Alves, Agnelo Neves, Sousa, Kaline de Brito, Bussadori, Sandra Kalil, Hamblin, Michael R., Nunes, Fábio Daumas
• Format: Article
• Language: English
• Subjects: Animals; Cell Line; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Cytokines; Inflammatory markers; Interleukin-6 - genetics; Interleukin-6 - metabolism; J774 cells; Lasers; LLLT; M1-activated macrophages; Macrophages - cytology; Macrophages - metabolism; Macrophages - radiation effects; Mice; Mice, Inbred BALB C; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Photobiomodulation; RNA, Messenger - metabolism; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2015.10.015

M1 profile macrophages exert a major influence on initial tissue repair process. Few days after the occurrence of injury, macrophages in the injured region exhibit a M2 profile, attenuate the effects of the M1 population, and stimulate the reconstruction of the damaged tissue. The different effects of macrophages in the healing process suggest that these cells could be the target of therapeutic interventions. Photobiomodulation has been used to accelerate tissue repair, but little is known regarding its effect on macrophages. In the present study, J774 macrophages were activated to simulate the M1 profile and irradiated with two different sets of laser parameters (780nm, 70mW, 2.6J/cm2, 1.5s and 660nm, 15mW, 7.5J/cm2, 20s). IL-6, TNF-α, iNOS and COX-2 gene and protein expression were analyzed by RT-qPCR and ELISA. Both lasers were able to reduce TNF-α and iNOS expression, and TNF-α and COX-2 production, although the parameters used for 780nm laser provided an additional decrease. 660nm laser parameters resulted in an up-regulation of IL-6 expression and production. These findings imply a distinct, time-dependent modulation by the two different sets of laser parameters, suggesting that the best modulation may involve more than one combination of parameters. •M1 J774 macrophages were irradiated with two different sets of laser parameters.•IL-6, TNF-α, iNOS and COX-2 expression were analyzed by RT-qPCR and ELISA.•660nm laser reduced the expression of TNF-α, iNOS and COX-2 in M1 J774 macrophages.•660nm laser increased the expression of IL-6 in M1 J774 macrophages.•780nm laser reduced IL-6, TNF-α, iNOS and COX-2 expression in J774 macrophages.