Multimode fibre: Light-sheet microscopy at the tip of a needle

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a mu...

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Bibliographic Details
Published in:Scientific reports 2015-12, Vol.5 (1), p.18050-18050, Article 18050
Main Authors: Plöschner, Martin, Kollárová, Věra, Dostál, Zbyněk, Nylk, Jonathan, Barton-Owen, Thomas, Ferrier, David E. K., Chmelík, Radim, Dholakia, Kishan, Čižmár, Tomáš
Format: Article
Language:English
Subjects:
639/624/1075/187
639/624/1107/510
Equipment Design - methods
Fluorescence microscopy
Holography
Humanities and Social Sciences
Illumination
Imaging, Three-Dimensional - methods
Light
Lighting - methods
Microscopy
Microscopy, Fluorescence - methods
multidisciplinary
Needles
Operculum
Optical Devices
Optical Fibers
Science
Transport processes
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Summary:Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki .
ISSN:2045-2322
2045-2322
DOI:10.1038/srep18050