New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

The inhibition of α -glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n -butan...

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Bibliographic Details
Published in:Natural products and bioprospecting 2015-12, Vol.5 (6), p.277-285
Main Authors: Batista, Daniela, Falé, Pedro L., Serralheiro, Maria L., Araújo, Maria E., Madeira, Paulo J. A., Borges, Carlos, Torgal, Isabel, Goulart, Margarida, Justino, Jorge, Martins, Alice, Rauter, Amélia P.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Bioorganic Chemistry
Fabaceae
Food Science
Genista
Life Sciences
Medicinal Chemistry
Original
Original Article
Pharmacology/Toxicology
Plant Biochemistry
Plant Sciences
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Summary:The inhibition of α -glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n -butanol extract from Genista tenera (Fabaceae). This extract decreased α -glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25 %, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06 %. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme (47.5 % inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher (67.82 %) than that promoted by ascorbic acid, the positive control (45.05 %). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC–DAD-ESI–MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices. Graphical Abstract
ISSN:2192-2195
2192-2209
DOI:10.1007/s13659-015-0077-z