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Staphylococcus aureus forms spreading dendrites that have characteristics of active motility
Staphylococcus aureus is historically regarded as a non-motile organism. More recently it has been shown that S. aureus can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites:...
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Published in: | Scientific reports 2015-12, Vol.5 (1), p.17698-17698, Article 17698 |
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description | Staphylococcus aureus
is historically regarded as a non-motile organism. More recently it has been shown that
S. aureus
can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that
S. aureus
can spread across the surface of media in structures that we term ‘comets’, which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of
S. aureus
isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other
S. aureus
cells behind them forming a comet ‘tail’; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that
S. aureus
is actively motile under certain conditions. |
doi_str_mv | 10.1038/srep17698 |
format | article |
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is historically regarded as a non-motile organism. More recently it has been shown that
S. aureus
can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that
S. aureus
can spread across the surface of media in structures that we term ‘comets’, which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of
S. aureus
isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other
S. aureus
cells behind them forming a comet ‘tail’; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that
S. aureus
is actively motile under certain conditions.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep17698</identifier><identifier>PMID: 26680153</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/28 ; 631/326/1320 ; 631/326/171 ; Agar ; Comets ; Dendrites ; Gram-positive bacteria ; Humanities and Social Sciences ; Microscopy, Electron, Scanning ; Microscopy, Phase-Contrast ; Motility ; Movement - physiology ; multidisciplinary ; Science ; Spreading ; Staphylococcus aureus ; Staphylococcus aureus - physiology ; Surface-Active Agents - metabolism ; Time-Lapse Imaging</subject><ispartof>Scientific reports, 2015-12, Vol.5 (1), p.17698-17698, Article 17698</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Dec 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-14deb44772b16b7d4b02219cd851b2cf6ca590c1df0708b6953db7abb6ded29b3</citedby><cites>FETCH-LOGICAL-c438t-14deb44772b16b7d4b02219cd851b2cf6ca590c1df0708b6953db7abb6ded29b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1800115455/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1800115455?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,36992,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26680153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pollitt, Eric J. G.</creatorcontrib><creatorcontrib>Crusz, Shanika A.</creatorcontrib><creatorcontrib>Diggle, Stephen P.</creatorcontrib><title>Staphylococcus aureus forms spreading dendrites that have characteristics of active motility</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Staphylococcus aureus
is historically regarded as a non-motile organism. More recently it has been shown that
S. aureus
can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that
S. aureus
can spread across the surface of media in structures that we term ‘comets’, which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of
S. aureus
isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other
S. aureus
cells behind them forming a comet ‘tail’; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that
S. aureus
is actively motile under certain conditions.</description><subject>14/28</subject><subject>631/326/1320</subject><subject>631/326/171</subject><subject>Agar</subject><subject>Comets</subject><subject>Dendrites</subject><subject>Gram-positive bacteria</subject><subject>Humanities and Social Sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Phase-Contrast</subject><subject>Motility</subject><subject>Movement - physiology</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Spreading</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - physiology</subject><subject>Surface-Active Agents - metabolism</subject><subject>Time-Lapse Imaging</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNplkctKxDAUhoMoKurCF5CCGxVGkzRJ040g4g0EF-pOCLnVRtqmJqkwb29kdBg1m5NwPv5zwgfAPoKnCJb8LAY7oorVfA1sY0joDJcYr6_ct8BejG8wH4prgupNsIUZ4xDRchu8PCY5tvPOa6_1FAs5BZtL40MfizgGK40bXgtjBxNcsrFIrUxFKz9soVsZpE42uJicjoVvivx0udP75DqX5rtgo5FdtHvfdQc8X189Xd7O7h9u7i4v7mealDzNEDFWEVJVWCGmKkMUxBjV2nCKFNYN05LWUCPTwApyxWpaGlVJpZixBteq3AHni9xxUr012g4pyE6MwfUyzIWXTvzuDK4Vr_5DEMZLWuIccPQdEPz7ZGMSvYvadp0crJ-iQBWFhCDGq4we_kHf_BSG_D2BOIQIUUJppo4XlA4-ZkPNchkExZc2sdSW2YPV7Zfkj6QMnCyA7CPLsGFl5L-0T6UDo_o</recordid><startdate>20151218</startdate><enddate>20151218</enddate><creator>Pollitt, Eric J. 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G. ; Crusz, Shanika A. ; Diggle, Stephen P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-14deb44772b16b7d4b02219cd851b2cf6ca590c1df0708b6953db7abb6ded29b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>14/28</topic><topic>631/326/1320</topic><topic>631/326/171</topic><topic>Agar</topic><topic>Comets</topic><topic>Dendrites</topic><topic>Gram-positive bacteria</topic><topic>Humanities and Social Sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Phase-Contrast</topic><topic>Motility</topic><topic>Movement - physiology</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Spreading</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - physiology</topic><topic>Surface-Active Agents - metabolism</topic><topic>Time-Lapse Imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pollitt, Eric J. G.</creatorcontrib><creatorcontrib>Crusz, Shanika A.</creatorcontrib><creatorcontrib>Diggle, Stephen P.</creatorcontrib><collection>Springer_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pollitt, Eric J. G.</au><au>Crusz, Shanika A.</au><au>Diggle, Stephen P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcus aureus forms spreading dendrites that have characteristics of active motility</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-12-18</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>17698</spage><epage>17698</epage><pages>17698-17698</pages><artnum>17698</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Staphylococcus aureus
is historically regarded as a non-motile organism. More recently it has been shown that
S. aureus
can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that
S. aureus
can spread across the surface of media in structures that we term ‘comets’, which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of
S. aureus
isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other
S. aureus
cells behind them forming a comet ‘tail’; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that
S. aureus
is actively motile under certain conditions.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26680153</pmid><doi>10.1038/srep17698</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 14/28 631/326/1320 631/326/171 Agar Comets Dendrites Gram-positive bacteria Humanities and Social Sciences Microscopy, Electron, Scanning Microscopy, Phase-Contrast Motility Movement - physiology multidisciplinary Science Spreading Staphylococcus aureus Staphylococcus aureus - physiology Surface-Active Agents - metabolism Time-Lapse Imaging |
title | Staphylococcus aureus forms spreading dendrites that have characteristics of active motility |
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