Stable kinetochore–microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells

• Published in: Nature communications 2015-12, Vol.6 (1), p.10036-10036, Article 10036
• Main Authors: Tauchman, Eric C., Boehm, Frederick J., DeLuca, Jennifer G.
• Format: Article
• Language: English
• Subjects: 13/1; 13/106; 13/109; 13/89; 14/1; 14/19; 14/35; 14/63; 631/80/128/1653; 631/80/641/1966; 631/80/641/2187; 631/80/86; Chromosome Segregation; Chromosomes; Cytoskeletal Proteins; HeLa Cells; Humanities and Social Sciences; Humans; Kinases; Kinetochores - metabolism; M Phase Cell Cycle Checkpoints; Microtubules - genetics; Microtubules - metabolism; Mitosis; Molecular biology; multidisciplinary; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Phosphorylation; Proteins; Science; Science (multidisciplinary); Spindle Apparatus - genetics; Spindle Apparatus - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms10036

During mitosis, duplicated sister chromatids attach to microtubules emanating from opposing sides of the bipolar spindle through large protein complexes called kinetochores. In the absence of stable kinetochore–microtubule attachments, a cell surveillance mechanism known as the spindle assembly checkpoint (SAC) produces an inhibitory signal that prevents anaphase onset. Precisely how the inhibitory SAC signal is extinguished in response to microtubule attachment remains unresolved. To address this, we induced formation of hyper-stable kinetochore–microtubule attachments in human cells using a non-phosphorylatable version of the protein Hec1, a core component of the attachment machinery. We find that stable attachments are sufficient to silence the SAC in the absence of sister kinetochore bi-orientation and strikingly in the absence of detectable microtubule pulling forces or tension. Furthermore, we find that SAC satisfaction occurs despite the absence of large changes in intra-kinetochore distance, suggesting that substantial kinetochore stretching is not required for quenching the SAC signal. The spindle assembly checkpoint prevents mitotic progression when chromosomes are not properly attached to the mitotic spindle. Here Tauchman et al. show that stable microtubule attachment to the kinetochore, and not tension generated from this interaction, is sufficient to silence the checkpoint.