Seven Direct Methods for Measuring HDL and LDL Cholesterol Compared with Ultracentrifugation Reference Measurement Procedures

Methods from 7 manufacturers and 1 distributor for directly measuring HDL cholesterol (C) and LDL-C were evaluated for imprecision, trueness, total error, and specificity in nonfrozen serum samples. We performed each direct method according to the manufacturer's instructions, using a Roche/Hita...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2010-06, Vol.56 (6), p.977-986
Main Authors: MILLER, W. Greg, MYERS, Gary L, NAKAJIMA, Katsuyuki, NAKAMURA, Masakazu, NILSSON, Göran, SHAMBUREK, Robert D, VETROVEC, George W, RUSSELL WARNICK, G, REMALEY, Alan T, SAKURABAYASHI, Ikunosuke, BACHMANN, Lorin M, CAUDILL, Samuel P, DZIEKONSKI, Andrzej, EDWARDS, Selvin, KIMBERLY, Mary M, KORZUN, William J, LEARY, Elizabeth T
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blood Chemical Analysis - methods
Cardiovascular disease
Cholesterol
Cholesterol, HDL - blood
Cholesterol, LDL - blood
Disease control
Fundamental and applied biological sciences. Psychology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Lipoproteins
Medical sciences
Methods
Molecular biophysics
Reference Standards
Risk assessment
Sensitivity and Specificity
Shipments
Ultracentrifugation
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Summary:Methods from 7 manufacturers and 1 distributor for directly measuring HDL cholesterol (C) and LDL-C were evaluated for imprecision, trueness, total error, and specificity in nonfrozen serum samples. We performed each direct method according to the manufacturer's instructions, using a Roche/Hitachi 917 analyzer, and compared the results with those obtained with reference measurement procedures for HDL-C and LDL-C. Imprecision was estimated for 35 runs performed with frozen pooled serum specimens and triplicate measurements on each individual sample. Sera from 37 individuals without disease and 138 with disease (primarily dyslipidemic and cardiovascular) were measured by each method. Trueness and total error were evaluated from the difference between the direct methods and reference measurement procedures. Specificity was evaluated from the dispersion in differences observed. Imprecision data based on 4 frozen serum pools showed total CVs
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2009.142810