Hybrid polymeric hydrogels via peptide nucleic acid (PNA)/DNA complexation

This work presents a new concept in hybrid hydrogel design. Synthetic water-soluble N-(2-hydroxypropyl)methacrylamide (HPMA) polymers grafted with multiple peptide nucleic acids (PNAs) are crosslinked upon addition of the linker DNA. The self-assembly is mediated by the PNA–DNA complexation, which r...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2015-12, Vol.220 (Pt B), p.608-616
Main Authors: Chu, Te-Wei, Feng, Jiayue, Yang, Jiyuan, Kopeček, Jindřich
Format: Article
Language:English
Subjects:
Acrylamides - chemistry
Acrylamides - metabolism
DNA
DNA - chemistry
DNA - metabolism
DNA - ultrastructure
Elasticity
Gene Transfer Techniques
HPMA polymer
Hydrogel
Hydrogels
Hydrophobic and Hydrophilic Interactions
Microscopy, Atomic Force
Nucleic Acid Conformation
P-form
Peptide nucleic acid
Peptide Nucleic Acids - chemistry
Peptide Nucleic Acids - metabolism
Peptide Nucleic Acids - ultrastructure
Porosity
Protein Conformation
Rheology
Self-assembly
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This work presents a new concept in hybrid hydrogel design. Synthetic water-soluble N-(2-hydroxypropyl)methacrylamide (HPMA) polymers grafted with multiple peptide nucleic acids (PNAs) are crosslinked upon addition of the linker DNA. The self-assembly is mediated by the PNA–DNA complexation, which results in the formation of hydrophilic polymer networks. We show that the hydrogels can be produced through two different types of complexations. Type I hydrogel is formed via the PNA/DNA double-helix hybridization. Type II hydrogel utilizes a unique “P-form” oligonucleotide triple-helix that comprises two PNA sequences and one DNA. Microrheology studies confirm the respective gelation processes and disclose a higher critical gelation concentration for the type I gel when compared to the type II design. Scanning electron microscopy reveals the interconnected microporous structure of both types of hydrogels. Type I double-helix hydrogel exhibits larger pore sizes than type II triple-helix gel. The latter apparently contains denser structure and displays greater elasticity as well. The designed hybrid hydrogels have potential as novel biomaterials for pharmaceutical and biomedical applications. [Display omitted]
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2015.09.035