Correlational research of Golgi phosphorylation protein 3 expression in colorectal cancer

• Published in: World journal of gastroenterology : WJG 2015-12, Vol.21 (48), p.13473-13479
• Main Authors: Guo, Yan-Ta, Qiu, Cheng-Zhi, Huang, Zhong-Xin, Yu, Wai-Shi, Yang, Xiao-Feng, Wang, Ming-Zhen
• Format: Article
• Language: English
• Subjects: Aged; Antigens, CD34 - analysis; Apoptosis; Basic Study; Biomarkers, Tumor - analysis; Colorectal Neoplasms - blood supply; Colorectal Neoplasms - chemistry; Colorectal Neoplasms - mortality; Colorectal Neoplasms - pathology; Female; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Male; Membrane Proteins - analysis; Microvessels - chemistry; Microvessels - pathology; Middle Aged; Neoplasm Staging; Neovascularization, Pathologic; Time Factors; Up-Regulation; Vascular Endothelial Growth Factor A - analysis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v21.i48.13473

To investigate the effect of Golgi phosphorylation protein 3 (GOLPH3) expression on cell apoptosis, angiogenesis and prognosis in colorectal cancer (CRC). The expression of GOLPH3 in CRC tissues and normal colorectal mucosae was determined by immunohistochemistry in 62 patients. In addition, immunohistochemistry was also carried out to detect the expression of vascular endothelial growth factor (VEGF), CD34 and microvessel density (MVD). Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay was used to determine the apoptotic index (AI). The Kaplan-Meier method was used to analyze the relationship between GOLPH3 expression and survival in another 123 CRC cases. Compared with normal colorectal mucosae, a notably higher level of GOLPH3 protein expression was identified in CRC tissues (53.2% vs 24.2%, P < 0.05). Positive GOLPH3 expression was significantly associated with tumor invasion depth, TNM stage, and lymph node metastasis (P = 0.001; P = 0.020; P = 0.020; P < 0.05, respectively), but not with tumor length, tumor site, and age (P = 0.363; P = 0.819; P = 0.599; P > 0.05, respectively). VEGF expression and MVD in GOLPH3-positive CRC was significantly higher than in GOLPH3-negative CRC (VEGF: 69.7% vs 31.0%; MVD: 21.45 ± 9.39 vs 14.24 ± 8.97; P < 0.05). GOLPH3 expression was negatively correlated with AI in CRC as shown by Spearman correlation analysis (r = -0.320, P < 0.05). The 5-year survival rate in GOLPH3-negative CRC (69.4%) was significantly higher than in GOLPH3-positive CRC (48.6%) (log-rank test, P < 0.05). High expression of GOLPH3 is found in CRC tissues. GOLPH3 expression may be a novel prognostic marker for CRC patients.