The efficiency of Poly(ADP-Ribose) Polymerase (PARP) cleavage on detection of apoptosis in an experimental model of testicular torsion

• Published in: International journal of experimental pathology 2015-10, Vol.96 (5), p.294-300
• Main Authors: Aslan Koşar, Pınar, Tuncer, Hamdi, Cihangir Uğuz, Abdülhadi, Espino Palma, Javier, Darıcı, Hakan, Onaran, İbrahim, Çiğ, Bilal, Koşar, Alim, Rodriguez Moratinos, Ana Beatriz
• Format: Article
• Language: English
• Subjects: Animals; apoptosis; Apoptosis - physiology; Blotting, Western; Disease Models, Animal; Functional Laterality - physiology; Immunohistochemistry; Male; Original; poly(ADP-ribose) polymerase (PARP); Poly(ADP-ribose) Polymerases - metabolism; Rats; Rats, Wistar; Spermatic Cord Torsion - enzymology; Spermatic Cord Torsion - pathology; Spermatozoa - pathology; testis torsion
• ISSN: 0959-9673; 1365-2613
• DOI: 10.1111/iep.12137

Summary The aim of this study was to evaluate the histopathological and apoptotic changes occurring in the rat ipsilateral and contralateral testes, after experimental spermatic cord torsion, and to explore and the role of poly(ADP‐ribose) polymerase (PARP) cleavage in testicular torsion–detorsion injury. A total of 37 Wistar albino rats were subjected to 720° unilateral spermatic cord torsion for 1, 2 and 4 h, followed by 4‐h reperfusion, or else to a sham operation (control group). Histology of the testicle was evaluated using haematoxylin–eosin (H&E) staining and Johnsen's scoring system. Germ cell apoptosis was evaluated via active caspase‐3 immunostaining, and PARP expression levels were evaluated via Western blotting. The mean Johnsen's tubular biopsy scores (JTBS) of the ipsilateral testicles were lower for all torsion groups than for the controls (P