Apoptosis, proliferation and p53 gene expression of H. pylori associated gastric epithelial lesions

• Published in: World journal of gastroenterology : WJG 2001-12, Vol.7 (6), p.779-782
• Main Authors: Zhang, Z, Yuan, Y, Gao, H, Dong, M, Wang, L, Gong, Y H
• Format: Article
• Language: English
• Subjects: Apoptosis; Cell Division; Gastric Mucosa - pathology; Gene Expression; Genes, p53 - physiology; Helicobacter Infections - complications; Helicobacter pylori; Humans; Original Research; Stomach Diseases - genetics; Stomach Diseases - microbiology; Stomach Diseases - pathology; Stomach Diseases - physiopathology
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v7.i6.779

To study the relationship between Helicobacter pylori (H. pylori) and gastric carcinoma and its possible pathogenesis by H. pylori. DNEL technique and immunohistochemical technique were used to study the state of apoptosis, proliferation and p53 gene expression. A total of 100 gastric mucosal biopsy specimens, including 20 normal mucosa, 30 H. pylori-negative and 30 H. pylori-positive gastric precancerous lesions along with 20 gastric carcinomas were studied. There were several apoptotic cells in the superficial epithelium and a few proliferative cells within the neck of gastric glands, and no p53 protein expression in normal mucosa. In gastric carcinoma, there were few apoptotic cells, while there were a large number of proliferative cells, and expression of p53 protein significantly was increased. In the phase of metaplasia, the apoptotic index (AI, 4.36%+/-1.95%), proliferative index (PI, 19.11%+/-6.79%) and positivity of p53 expression (46.7%) in H. pylori-positive group were higher than those in normal mucosa (P