The effect of glycosylation on cytotoxicity of Ibaraki virus nonstructural protein NS3

The cytotoxicity of Ibaraki virus nonstructural protein NS3 was confirmed, and the contribution of glycosylation to this activity was examined by using glycosylation mutants of NS3 generated by site-directed mutagenesis. The expression of NS3 resulted in leakage of lactate dehydrogenase to the cultu...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2015, Vol.77(12), pp.1611-1616
Main Authors: URATA, Maho, WATANABE, Rie, IWATA, Hiroyuki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Arthropoda
Cloning, Molecular
Cytopathogenic Effect, Viral
cytotoxicity
Gene Expression Regulation, Viral - physiology
Glycosylation
HEK293 Cells
Humans
Ibaraki virus
intracellular distribution
Molecular Sequence Data
Mutagenesis, Site-Directed
NS3 glycosylation
Orbivirus
Orbivirus - physiology
Protein Structure, Tertiary
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - metabolism
Virology
Virus Internalization
Virus Replication
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Summary:The cytotoxicity of Ibaraki virus nonstructural protein NS3 was confirmed, and the contribution of glycosylation to this activity was examined by using glycosylation mutants of NS3 generated by site-directed mutagenesis. The expression of NS3 resulted in leakage of lactate dehydrogenase to the culture supernatant, suggesting the cytotoxicity of this protein. The lack of glycosylation impaired the transport of NS3 to the plasma membrane and resulted in reduced cytotoxicity. Combined with the previous observation that NS3 glycosylation was specifically observed in mammalian cells (Urata et al., Virus Research 2014), it was suggested that the alteration of NS3 cytotoxicity through modulating glycosylation is one of the strategies to achieve host specific pathogenisity of Ibaraki virus between mammals and vector arthropods.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.15-0121