Quantitative PCR detection of feline morbillivirus in cat urine samples

Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2015, Vol.77(12), pp.1701-1703
Main Authors: FURUYA, Tetsuya, WACHI, Akiko, SASSA, Yukiko, OMATSU, Tsutomu, NAGAI, Makoto, FUKUSHIMA, Ryuji, SHIBUTANI, Makoto, YAMAGUCHI, Tomohiro, UEMATSU, Yosuke, SHIROTA, Kinji, MIZUTANI, Tetsuya
Format: Article
Language:English
Subjects:
Animals
Cat Diseases - urine
Cat Diseases - virology
Cats
detection
feline
Morbillivirus
Morbillivirus - isolation & purification
Morbillivirus Infections - urine
Morbillivirus Infections - veterinary
Morbillivirus Infections - virology
paramyxovirus
PCR
Reverse Transcriptase Polymerase Chain Reaction - methods
Reverse Transcriptase Polymerase Chain Reaction - veterinary
Virology
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Summary:Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.15-0112