Isolation and Transmission of the Lyme Disease Spirochete from the Southeastern United States

The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-08, Vol.90 (15), p.7371-7375
Main Authors: Oliver, J. H., Chandler, F. W., Luttrell, M. P., James, A. M., Stallknecht, D. E., McGuire, B. S., Hutcheson, H. J., Cummins, G. A., Lane, R. S.
Format: Article
Language:English
Subjects:
Animals
Animals, Wild - microbiology
Antibodies
Antigens, Surface - genetics
Arachnid Vectors - microbiology
Bacterial Outer Membrane Proteins - genetics
Bacterial Vaccines
Bacteriology
Base Sequence
Biological and medical sciences
Borrelia burgdorferi
Borrelia burgdorferi Group - isolation & purification
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Gels
Georgia
Hamsters
Ixodes scapularis
Larvae
Lipoproteins
Lyme disease
Lyme Disease - microbiology
Medical research
Mice
Microbiology
Molecular Sequence Data
Nymphs
Oligodeoxyribonucleotides - chemistry
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Peromyscus - microbiology
Peromyscus gossypinus
Polymerase Chain Reaction
Spirochaetales
Ticks
Ticks - microbiology
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Summary:The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.15.7371