Silencing of Foxp3 delays the growth of murine melanomas and modifies the tumor immunosuppressive environment

Forkhead box p3 (Foxp3) expression was believed to be specific for T-regulatory cells but has recently been described in non-hematopoietic cells from different tissue origins and in tumor cells from both epithelial and non-epithelial tissues. The aim of this study was to elucidate the role of Foxp3...

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Published in:OncoTargets and therapy 2016-01, Vol.9, p.243-253
Main Authors: Franco-Molina, Moisés A, Miranda-Hernández, Diana F, Mendoza-Gamboa, Edgar, Zapata-Benavides, Pablo, Coronado-Cerda, Erika E, Sierra-Rivera, Crystel A, Saavedra-Alonso, Santiago, Taméz-Guerra, Reyes S, Rodríguez-Padilla, Cristina
Format: Article
Language:English
Subjects:
Cancer
Care and treatment
Cloning
Deoxyribonucleic acid
Development and progression
DNA
Dosage and administration
Enzyme-linked immunosorbent assay
Gene expression
Genetic aspects
Genotype & phenotype
Health aspects
Immunosuppressive agents
Lymphocytes
Melanoma
Metastasis
Nitrogen
Original Research
Plasmids
Polymerase chain reaction
Proteins
Transcription factors
Tumors
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Summary:Forkhead box p3 (Foxp3) expression was believed to be specific for T-regulatory cells but has recently been described in non-hematopoietic cells from different tissue origins and in tumor cells from both epithelial and non-epithelial tissues. The aim of this study was to elucidate the role of Foxp3 in murine melanoma. The B16F10 cell line Foxp3 silenced with small interference Foxp3 plasmid transfection was established and named B16F10.1. These cells had lower levels of Foxp3 mRNA (quantitative real-time reverse transcription-polymerase chain reaction [0.235-fold]), protein (flow cytometry [0.02%]), CD25(+) expression (0.06%), cellular proliferation (trypan blue staining), and interleukin (IL)-2 production (enzyme-linked immunosorbent assay [72.35 pg/mL]) than those in B16F10 wild-type (WT) cells (P
ISSN:1178-6930
1178-6930
DOI:10.2147/ott.s90476