Cooperative inhibitory effects of antisense oligonucleotide of cell adhesion molecules and cimetidine on cancer cell adhesion

• Published in: World journal of gastroenterology : WJG 2004, Vol.10 (1), p.62-66
• Main Authors: Tang, Nan-Hong, Chen, Yan-Ling, Wang, Xiao-Qian, Li, Xiu-Jin, Yin, Feng-Zhi, Wang, Xiao-Zhong
• Format: Article
• Language: English
• Subjects: Cell Adhesion - drug effects; Cell Line, Tumor; Cimetidine - pharmacology; Colorectal Neoplasms; E-Selectin - genetics; Electrophoresis, Agar Gel; Endothelium - cytology; Gene Expression; Histamine H2 Antagonists - pharmacology; Humans; Intercellular Adhesion Molecule-1 - genetics; Liver Cancer; Liver Neoplasms; Oligonucleotides, Antisense - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; 反义核苷酸; 甲氰咪胍; 细胞因子; 细胞粘附分子; 肿瘤抑制; 肿瘤细胞
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i1.62

AIM: To explore the cooperative effects of antisense oligonucleotide (ASON) of cell adhesion molecules and cimetidine on the expression of E-selectin and ICAM-1 in endothelial cells and their adhesion to tumor cells. METHODS: After treatment of endothelial cells with ASON and/or cimetidine and induction with TNF-α, the protein and mRNA changes of E-selectin and ICAM-1 in endothelial cells were examined by flow cytometry and RT-PCR,respectively. The adhesion rates of endothelial cells to tumor cells were measured by cell adhesion experiment. RESULTS: In comparison with TNF-α inducing group, lipoASON and lipo-ASON/cimetidine could significantly decrease the protein and mRNA levels of E-selectin and ICAM-1 in endothelial cells, and lipo-ASON/cimetidine had most significant inhibitory effect on E-selectin expression (from 36.37±1.56% to 14.23±1.07%, P