Selenium Supplementation of Amaranth Sprouts Influences Betacyanin Content and Improves Anti-Inflammatory Properties via NFκB in Murine RAW 264.7 Macrophages

• Published in: Biological trace element research 2016-02, Vol.169 (2), p.320-330
• Main Authors: Tyszka-Czochara, Malgorzata, Pasko, Pawel, Zagrodzki, Pawel, Gajdzik, Ewelina, Wietecha-Posluszny, Renata, Gorinstein, Shela
• Format: Article
• Language: English
• Subjects: adverse effects; amaranth grain; Amaranthus - chemistry; Amaranthus - growth & development; Amaranthus cruentus; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; antineoplastic agents; betacyanins; Betacyanins - isolation & purification; Betacyanins - pharmacology; Biochemistry; Biomedical and Life Sciences; Biotechnology; cell culture; Cell Line; cell nucleus; Cell Survival; feed supplements; interleukin-6; Interleukin-6 - immunology; Interleukin-6 - metabolism; Life Sciences; macrophages; Macrophages - drug effects; Macrophages - immunology; methanol; Mice; NF-kappa B - immunology; NF-kappa B - metabolism; Nutrition; nutritive value; Oncology; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Protein Transport; Seedlings - chemistry; Seedlings - growth & development; seeds; Seeds - chemistry; Seeds - growth & development; selenium; Selenium - isolation & purification; Selenium - pharmacology; sodium selenite; transcription factor NF-kappa B; viability
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-015-0429-x

Sprouts contain potent compounds which while influencing crucial transduction pathways in cell reveal anti-inflammatory and anticancer activities. In this study, we report the biological activity for seeds and colourful sprouts of four types of edible amaranth, as amaranth has recently attracted interest due to its appreciable nutritional value. MTT assay conducted for the amaranth seeds and sprouts did not show any adverse effect on the viability of murine RAW 264.7 cells. As amaranth accumulates selenium, the sprouts were supplemented with this trace element (10 mg/L; 15 mg/L Se as sodium selenite) while growing. Selenium concentration in sprouts was observed to be significantly correlated with betacyanins content of the tested species. The amounts of Se and betacyanins in sprouts varied for various Amaranth species. In the present study, Amaranthus cruentus sprouts with the highest betacyanins (19.30 ± 0.57–28.85 ± 2.23 mg of amaranthin/100 g of fresh weight) and high total selenium (22.51 ± 1.57–1044.75 ± 73.08 μg/L in methanol extracts) content prevented NFκB translocation to the cell nucleus and subsequently exerted an anti-inflammatory effect by significant decreasing inflammatory interleukin 6 production (587.3 ± 34.2–710.0 ± 88.1 pg/mL) in the cell culture of activated RAW 264.7 macrophages (vs LPS control 1520 ± 114 pg/mL).