Doxorubicin loaded nanodiamond-silk spheres for fluorescence tracking and controlled drug release

• Published in: Biomedical optics express 2016-01, Vol.7 (1), p.132-147
• Main Authors: Khalid, Asma, Mitropoulos, Alexander N, Marelli, Benedetto, Tomljenovic-Hanic, Snjezana, Omenetto, Fiorenzo G
• Format: Article
• Language: English
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/BOE.7.000132

Nanoparticle (NP) based technologies have proved to be considerably beneficial for advances in biomedicine especially in the areas of disease detection, drug delivery and bioimaging. Over the last few decades, NPs have garnered interest for their exemplary impacts on the detection, treatment, and prevention of cancer. The full potential of these technologies are yet to be employed for clinical use. The ongoing research and development in this field demands single multifunctional composite materials that can be employed simultaneously for drug delivery and biomedical imaging. In this manuscript, a unique combination of silk fibroin (SF) and nanodiamonds (NDs) in the form of nanospheres are fabricated and investigated. The spheres were loaded with the anthracyline Doxorubicin (DoX) and the drug release kinetics for these ND-SF-DoX (NDSX) spheres were studied. NDs provided the fluorescence modality for imaging while the degradable SF spheres stabilized and released the drug in a controlled manner. The emission and structural properties of the spheres were characterized during drug release. The degradability of SF and the subsequent release of DoX from the spheres were monitored through fluorescence of NDs inside the spheres. This research demonstrates the enormous potential of the ND-SF nanocomposite platforms for diagnostic and therapeutic purposes, which are both important for pharmaceutical research and clinical settings.