Plasma Lipid Profiling in a Rat Model of Hepatocellular Carcinoma: Potential Modulation Through Quinolone Administration

Background/Aims The primary aim of this study was to characterise the blood metabolic profile of hepatocellular carcinoma (HCC) in a rat model, and the secondary aim was to evaluate the effect of the quinolone, norfloxacin on metabolic profiles and exploring the role that gut sterilisation may have...

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Published in:Journal of clinical and experimental hepatology 2015-12, Vol.5 (4), p.286-294
Main Authors: Shariff, Mohamed I.F, Tognarelli, Joshua M, Lewis, Matthew R, Want, Elizabeth J, Mohamed, Fatma El Zahra, Ladep, Nimzing G, Crossey, Mary M.E, Khan, Shahid A, Jalan, Rajiv, Holmes, Elaine, Taylor-Robinson, Simon D
Format: Article
Language:English
Subjects:
Endocrinology & Metabolism
Gastroenterology and Hepatology
hepatocellular carcinoma
metabonomics
NMR spectroscopy
norfloxacin
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Summary:Background/Aims The primary aim of this study was to characterise the blood metabolic profile of hepatocellular carcinoma (HCC) in a rat model, and the secondary aim was to evaluate the effect of the quinolone, norfloxacin on metabolic profiles and exploring the role that gut sterilisation may have on HCC development. Methods HCC was induced in 10 Fischer rats by administration of intra-peritoneal diethylnitrosamine (DEN) and oral N-nitrosomorpholine. Plasma was collected upon sacrifice. Five of these rats were concomitantly administered oral norfloxacin. Six Fischer non-treated rats acted as healthy controls. Proton nuclear magnetic resonance (NMR) spectra were acquired using a 600 MHz NMR system. Results Control animals were 120 g heavier than diseased counterparts. Proton NMR spectra from diseased rats displayed significant decreases in lipoproteins, unsaturated fatty acids, acetyl-glycoprotein, acetoacetate, and glucose ( P ≤ 0.001). Plasma citrate and formate levels were increased ( P = 0.02). Norfloxacin appeared to abrogate this effect slightly. Conclusion The spectral profiles of plasma in rats with HCC display marked changes with relation to lipid metabolism and cellular turnover. Norfloxacin appears to moderate these metabolic alterations to a small degree.
ISSN:0973-6883
2213-3453
DOI:10.1016/j.jceh.2015.07.205