Effect of antisense oligodeoxynucleotide of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer

AIM:To explore the effect of antisense oligocleoxynucletide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.RESULTS:The telomerase activity in SW480 cells transfected with 1.0μmol/L of As-ODN for 2-5days, was significantly decreased in a time-dependent mann...

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Published in:World journal of gastroenterology : WJG 2004-02, Vol.10 (3), p.443-445
Main Authors: Jiang, Ying-An, Luo, He-Sheng, Fan, Li-Fang, Jiang, Chong-Qing, Chen, Wei-Jin
Format: Article
Language:English
Subjects:
Brief Reports
Cell Line, Tumor
Colonic Neoplasms - enzymology
Colonic Neoplasms - physiopathology
Humans
Oligodeoxyribonucleotides, Antisense - pharmacology
RNA - genetics
Telomerase - genetics
Telomerase - metabolism
反义寡核苷酸
基因疗法
端粒酶
细胞凋亡
结肠癌
酶联免疫吸附法
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Summary:AIM:To explore the effect of antisense oligocleoxynucletide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.RESULTS:The telomerase activity in SW480 cells transfected with 1.0μmol/L of As-ODN for 2-5days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis.The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes.CONCLUSION:As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v10.i3.443