Quantification of the Metabolic State in Cell-Model of Parkinson’s Disease by Fluorescence Lifetime Imaging Microscopy

• Published in: Scientific reports 2016-01, Vol.6 (1), p.19145-19145, Article 19145
• Main Authors: Chakraborty, Sandeep, Nian, Fang-Shin, Tsai, Jin-Wu, Karmenyan, Artashes, Chiou, Arthur
• Format: Article
• Language: English
• Subjects: 631/1647/328/2057; 631/378/87; 631/57/2267; Adenine; Animals; Data processing; Energy Metabolism - drug effects; Enzymes; Flavin-adenine dinucleotide; Flavin-Adenine Dinucleotide - metabolism; Glycolysis; Humanities and Social Sciences; Metabolism; Metabolomics - methods; Microscopy; Microscopy, Fluorescence - methods; Models, Biological; Movement disorders; MPP; multidisciplinary; NAD; NAD - metabolism; NADH; Nerve growth factor; Nerve Growth Factor - pharmacology; Neurodegenerative diseases; Nicotinamide; Oxidative phosphorylation; Parkinson Disease - metabolism; Parkinson's disease; PC12 Cells; Pheochromocytoma cells; Phosphorylation; Proteins; Rats; Science; Statistical analysis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep19145

Intracellular endogenous fluorescent co-enzymes, reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), play a pivotal role in cellular metabolism; quantitative assessment of their presence in living cells can be exploited to monitor cellular energetics in Parkinson’s disease (PD), a neurodegenerative disorder. Here, we applied two-photon fluorescence lifetime imaging microscopy (2P-FLIM) to noninvasively measure the fluorescence lifetime components of NADH and FAD and their relative contributions in MPP + (1-methyl-4-phenylpyridinium) treated neuronal cells, derived from PC12 cells treated with nerve growth factor (NGF), to mimic PD conditions. A systematic FLIM data analysis showed a statistically significant (p