Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq

Telomerase is a ribonucleoprotein that maintains the ends of linear chromosomes in most eukaryotes. Loss of telomerase activity results in shortening of telomeric DNA and eventually a specific G2/M cell-cycle arrest known as senescence. In humans, telomere shortening occurs during aging, while inapp...

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Bibliographic Details
Published in:Scientific reports 2016-01, Vol.6 (1), p.19376-19376, Article 19376
Main Authors: Niederer, Rachel O., Papadopoulos, Nickolas, Zappulla, David C.
Format: Article
Language:English
Subjects:
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631/208/212/2019
631/337/384/2568
Aging
Cancer
Cellular Senescence
Chromosomes
Cluster Analysis
Deoxyribonucleic acid
DNA
Epistasis, Genetic
Gene Expression Profiling
Gene Expression Regulation, Fungal
Humanities and Social Sciences
multidisciplinary
Mutation
Non-coding RNA
Ribonucleic acid
RNA
RNA, Long Noncoding - genetics
RNA, Untranslated
Science
Senescence
Sequence Analysis, RNA
Telomerase
Telomerase - deficiency
Transcription
Transcription, Genetic
Yeast
Yeasts
Yeasts - genetics
Yeasts - metabolism
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Summary:Telomerase is a ribonucleoprotein that maintains the ends of linear chromosomes in most eukaryotes. Loss of telomerase activity results in shortening of telomeric DNA and eventually a specific G2/M cell-cycle arrest known as senescence. In humans, telomere shortening occurs during aging, while inappropriate activation of telomerase is associated with approximately 90% of cancers. Previous studies have identified several classes of noncoding RNAs (ncRNA) also associated with aging-related senescence and cancer, but whether ncRNAs are also involved in short-telomere-induced senescence in yeast is unknown. Here, we report 112 putative novel lncRNAs in the yeast Saccharomyces cerevisiae , 41 of which are only expressed in telomerase-negative yeast. Expression of approximately half of the lncRNAs is strongly correlated with that of adjacent genes, suggesting this subset may influence transcription of neighboring genes. Our results reveal a new potential mechanism governing adaptive changes in senescing and post-senescent survivor yeast cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep19376