Mesenchymal stem cells maintain their defining stem cell characteristics after treatment with cisplatin

Mesenchymal stem cells (MSCs) aid the regeneration of tissues damaged by treatment with cisplatin. However, the effects of this cytotoxic drug on the stem cells have been largely unknown. Here we demonstrate that human bone marrow-derived MSCs are relatively resistant to cisplatin treatment and show...

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Bibliographic Details
Published in:Scientific reports 2016-01, Vol.6 (1), p.20035-20035, Article 20035
Main Authors: Nicolay, Nils H., Perez, Ramon Lopez, Rühle, Alexander, Trinh, Thuy, Sisombath, Sonevisay, Weber, Klaus-Josef, Ho, Anthony D., Debus, Jürgen, Saffrich, Rainer, Huber, Peter E.
Format: Article
Language:English
Subjects:
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Apoptosis
Apoptosis - drug effects
Bone marrow
Bone Marrow Cells - cytology
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cell Proliferation - drug effects
Cell Proliferation - genetics
Cells, Cultured
Cisplatin
Cisplatin - administration & dosage
Cytoskeleton
Cytotoxicity
Drug Resistance - genetics
Fibroblasts
Fibroblasts - drug effects
Heat shock proteins
Humanities and Social Sciences
Humans
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchyme
multidisciplinary
Phenotype
Regeneration
Regeneration - drug effects
Science
Stem cells
Surface markers
Therapeutic applications
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Summary:Mesenchymal stem cells (MSCs) aid the regeneration of tissues damaged by treatment with cisplatin. However, the effects of this cytotoxic drug on the stem cells have been largely unknown. Here we demonstrate that human bone marrow-derived MSCs are relatively resistant to cisplatin treatment and show resistance levels comparable to these of differentiated fibroblasts. Cisplatin did not affect cellular morphology, adhesion or induction of apoptosis in MSCs. The potential for differentiation was preserved after exposure to cisplatin and established MSC surface markers were observed to be stably expressed irrespective of cisplatin treatment. Cytoskeletal rearrangements and high expression levels of individual heat shock proteins were detected in MSCs and may be partly responsible for the observed cisplatin resistance. The cisplatin-resistant phenotype of human MSCs supports the concept of further investigating these stem cells as a potential treatment option for cisplatin-induced tissue damage.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep20035