MicroRNA-147 suppresses proliferation, invasion and migration through the AKT/mTOR signaling pathway in breast cancer

The Akt/mTOR pathway is considered to be the most frequently aberrantly activated pathway in human tumors. It is activated in a variety of types of tumor, and is therefore an attractive target for study, including it's potential regulation by microRNAs. A number of microRNAs (miRs) have been de...

Full description

Saved in:
Bibliographic Details
Published in:Oncology letters 2016-01, Vol.11 (1), p.405-410
Main Authors: ZHANG, YULONG, ZHANG, HE, LIU, ZHE
Format: Article
Language:English
Subjects:
Akt/mTOR pathway
Breast cancer
Cancer therapies
Cell cycle
Cell growth
Cellular signal transduction
Colorectal cancer
Development and progression
Genetic aspects
Health aspects
invasion
Kinases
MicroRNA
migration
Motility
Oncology
Phosphorylation
proliferation
Studies
Tumorigenesis
Tumors
Vectors (Biology)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Akt/mTOR pathway is considered to be the most frequently aberrantly activated pathway in human tumors. It is activated in a variety of types of tumor, and is therefore an attractive target for study, including it's potential regulation by microRNAs. A number of microRNAs (miRs) have been demonstrated to target the Akt/mTOR pathway. A previous study reported that miR-147 targets the EGFR-driven cell-cycle protein network in breast cancer. EGFR serves a crucial role upstream to Akt/mTOR. To define the function and mechanism of miR-147 in breast cancer, the present study assessed miR-147 expression in a normal mammary epithelial cell line and three breast cancer cell lines, and observed that miR-147 expression was markedly low in the highly invasive cell line, MDA-MB-231. Ectopic expression of miR-147 in MDA-MB-231 resulted in a reduction of the phosphorylation of crucial molecules in the Akt/mTOR pathway and the proliferation, invasion and migration of the cell line was also reduced. The effects of miR-147 expression are similar to that of shRNA which is specifically designed to silence the expression of Akt. The findings of the present study indicate that miR-147 suppressed the proliferation, invasion and migration of breast cancer cells through targeting the Akt/mTOR signaling pathway. As a new microRNA targeting Akt/mTOR pathway. Using miR-147 may therefore provide an effective therapeutic approach to suppress tumorigenicity in breast cancer.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2015.3842