Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression

The present study demonstrates the effect of rubriflordilactone on lipopolysaccharide (LPS)-induced acute kidney injury in rats and MLE-15 cells. LPS administration in rats resulted in formation of edema which was inhibited by pretreatment with rubriflordilactone. The pulmonary tissues of LPS admini...

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Bibliographic Details
Published in:International journal of clinical and experimental pathology 2015-01, Vol.8 (12), p.15954-15959
Main Authors: Wang, Yan-Ying, Qiu, Xin-Guang, Ren, Hong-Liang
Format: Article
Language:English
Subjects:
Acute Lung Injury - chemically induced
Acute Lung Injury - metabolism
Acute Lung Injury - pathology
Acute Lung Injury - prevention & control
Animals
Anti-Inflammatory Agents - pharmacology
Cell Line
Disease Models, Animal
Dose-Response Relationship, Drug
Inflammation Mediators - metabolism
Interleukin-6 - metabolism
Lipopolysaccharides
Lung - drug effects
Lung - metabolism
Lung - pathology
Male
Matrix Metalloproteinase 9 - metabolism
Mice
Nitric Oxide Synthase Type II - metabolism
Original
Pulmonary Edema - chemically induced
Pulmonary Edema - metabolism
Pulmonary Edema - prevention & control
Rats, Wistar
RNA Interference
Sirtuin 1 - genetics
Sirtuin 1 - metabolism
Transfection
Triterpenes - pharmacology
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Summary:The present study demonstrates the effect of rubriflordilactone on lipopolysaccharide (LPS)-induced acute kidney injury in rats and MLE-15 cells. LPS administration in rats resulted in formation of edema which was inhibited by pretreatment with rubriflordilactone. The pulmonary tissues of LPS administered rats and MLE-15 cells showed a significant increase in the expression of matrix metalloproteinase-9, interleukin-6 and inducible nitric oxide synthase. However, rubriflordilactone treatment prior to LPS administration caused a significant reduction in the expression of these factors at a concentration of 10 nm/kg. Analysis of the Sirtuin 1 (Sirt1) expression revealed significant (P=0.002) reduction on exposure to LPS in MLE-15 cells. However, rubriflordilactone treatment at 10 nm/ml concentration before LPS exposure caused inhibition of LPS induced reduction in Sirt1 expression. Silencing of Sirt1 by siRNA in MLE-15 cells led to inhibition of increased Sirt1 expression by rubriflordilactone in LPS administered rats. These findings suggest that rubriflordilactone inhibits LPS induced acute lung injury in rats and MLE-15 cells through promotion of Sirt1 expression.
ISSN:1936-2625