Precursor and mature NGF live tracking: one versus many at a time in the axons

The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different and generally opposite biological functions with r...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-02, Vol.6 (1), p.20272-20272, Article 20272
Main Authors: De Nadai, Teresa, Marchetti, Laura, Di Rienzo, Carmine, Calvello, Mariantonietta, Signore, Giovanni, Di Matteo, Pierluigi, Gobbo, Francesco, Turturro, Sabrina, Meucci, Sandro, Viegi, Alessandro, Beltram, Fabio, Luin, Stefano, Cattaneo, Antonino
Format: Article
Language:English
Subjects:
13
13/62
14
14/34
631/337/2265
631/378
631/378/1686
631/57
82
82/83
Alzheimer's disease
Amino Acid Sequence
Animals
Axonal Transport
Axons
Axons - metabolism
Color
Dorsal root ganglia
Ganglia, Spinal
Gene expression
Growth factors
Humanities and Social Sciences
Humans
Labeling
Microfluidics
Models, Molecular
multidisciplinary
Nerve growth factor
Nerve Growth Factor - chemistry
Nerve Growth Factor - metabolism
Nervous system
Neurons - metabolism
Neurotrophic factors
Protein Conformation
Protein Interaction Domains and Motifs
Protein Multimerization
Protein Precursors - chemistry
Protein Precursors - metabolism
Proteins
Rats
Science
Transport Vesicles - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different and generally opposite biological functions with respect to its mature counterpart. Here we developed a strategy to fluorolabel both purified precursor and mature neurotrophins (NTs) with a controlled stoichiometry and insertion site. Using a single particle tracking approach, we characterized the axonal transport of proNGF versus mature NGF in living dorsal root ganglion neurons grown in compartmentalized microfluidic devices. We demonstrate that proNGF is retrogradely transported as NGF, but with a lower flux and a different distribution of numbers of neurotrophins per vesicle. Moreover, exploiting a dual-color labelling technique, we analysed the transport of both NT forms when simultaneously administered to the axon tips.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep20272