A Group of Novel Serum Diagnostic Biomarkers for Multidrug-Resistant Tuberculosis by iTRAQ-2D LC-MS/MS and Solexa Sequencing

The epidemic of pulmonary tuberculosis (TB), especially multidrug-resistance tuberculosis (MDR-TB) presented a major challenge for TB treatment today. We performed iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) and Solexa sequencing among MDR...

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Bibliographic Details
Published in:International journal of biological sciences 2016, Vol.12 (2), p.246-256
Main Authors: Wang, Chong, Liu, Chang-Ming, Wei, Li-Liang, Shi, Li-Ying, Pan, Zhi-Fen, Mao, Lian-Gen, Wan, Xiao-Chen, Ping, Ze-Peng, Jiang, Ting-Ting, Chen, Zhong-Liang, Li, Zhong-Jie, Li, Ji-Cheng
Format: Article
Language:English
Subjects:
Adult
Aged
Biomarkers - blood
Chromatography, Liquid
Data Mining
Decision Trees
Female
Humans
Male
MicroRNAs - blood
Middle Aged
Proteomics
Research Paper
ROC Curve
Sequence Analysis - methods
Tandem Mass Spectrometry
Transcriptome
Tuberculosis, Multidrug-Resistant - blood
Tuberculosis, Multidrug-Resistant - diagnosis
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Summary:The epidemic of pulmonary tuberculosis (TB), especially multidrug-resistance tuberculosis (MDR-TB) presented a major challenge for TB treatment today. We performed iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) and Solexa sequencing among MDR-TB patients, drug-sensitive tuberculosis (DS-TB) patients, and healthy controls. A total of 50 differentially expressed proteins and 43 differentially expressed miRNAs (fold change >1.50 or
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.13805