High resolution Physio-chemical Tissue Analysis: Towards Non-invasive In Vivo Biopsy

Conventional gold standard histopathologic diagnosis requires information of both high resolution structural and chemical changes in tissue. Providing optical information at ultrasonic resolution, photoacoustic (PA) technique could provide highly sensitive and highly accurate tissue characterization...

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Bibliographic Details
Published in:Scientific reports 2016-02, Vol.6 (1), p.16937-16937, Article 16937
Main Authors: Xu, Guan, Meng, Zhuo-xian, Lin, Jian-die, Deng, Cheri X., Carson, Paul L., Fowlkes, J. Brian, Tao, Chao, Liu, Xiaojun, Wang, Xueding
Format: Article
Language:English
Subjects:
639/624/1107/510
692/308/575
Analytical chemistry
Animals
Biopsy
Chemical composition
Collagen
Diet, High-Fat
Disease Models, Animal
Fatty liver
Fatty Liver - diagnostic imaging
Fatty Liver - metabolism
Fatty Liver - pathology
Fibrosis
Histology
Histopathology
Humanities and Social Sciences
Laboratory animals
Liver diseases
Mice
Mice, Inbred C57BL
Morphology
multidisciplinary
Photoacoustic Techniques
Science
Support Vector Machine
Tissues
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Summary:Conventional gold standard histopathologic diagnosis requires information of both high resolution structural and chemical changes in tissue. Providing optical information at ultrasonic resolution, photoacoustic (PA) technique could provide highly sensitive and highly accurate tissue characterization noninvasively in the authentic in vivo environment, offering a replacement for histopathology. A two-dimensional (2D) physio-chemical spectrogram (PCS) combining micrometer to centimeter morphology and chemical composition simultaneously can be generated for each biological sample with PA measurements at multiple optical wavelengths. This spectrogram presents a unique 2D “ physio-chemical signature ” for any specific type of tissue. Comprehensive analysis of PCS, termed PA physio-chemical analysis (PAPCA), can lead to very rich diagnostic information, including the contents of all relevant molecular and chemical components along with their corresponding histological microfeatures, comparable to those accessible by conventional histology. PAPCA could contribute to the diagnosis of many diseases involving diffusive patterns such as fatty liver.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep16937