Patterns of plasma cobalamins in control subjects and in cases of vitamin B12 deficiency

A method for thin-layer chromatography and bioautography of plasma cobalamins is described. It requires only 5 ml of blood. Results are reported in 35 healthy people and hospital controls, and in 14 cases of untreated pernicious anaemia. In the control group there were two major components, methylco...

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Bibliographic Details
Published in:Journal of clinical pathology 1969-09, Vol.22 (5), p.545-550
Main Authors: Linnell, J. C., Mackenzie, Heather M., Wilson, J., Matthews, D. M.
Format: Article
Language:English
Subjects:
Adult
Aged
Anemia, Pernicious - blood
Chromatography, Thin Layer
Diet, Vegetarian
Female
Humans
Male
Methods
Middle Aged
Smoking
Sunlight
Vitamin B 12 - blood
Vitamin B 12 Deficiency - blood
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Summary:A method for thin-layer chromatography and bioautography of plasma cobalamins is described. It requires only 5 ml of blood. Results are reported in 35 healthy people and hospital controls, and in 14 cases of untreated pernicious anaemia. In the control group there were two major components, methylcobalamin and a component which is probably a mixture of hydroxocobalamin and deoxyadenosyl cobalamin. Many cases of untreated pernicious anaemia showed a characteristic pattern in which methylcobalamin was reduced in relation to hydroxocobalamin. This pattern was also seen in one vegan who was taking a diet without B12 supplement. A minority of subjects showed traces of cyanocobalamin; this compound was not confined to smokers. The significance of the results and possible diagnostic utility of the technique are discussed.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.22.5.545