Human milk miRNAs primarily originate from the mammary gland resulting in unique miRNA profiles of fractionated milk

Human milk (HM) contains regulatory biomolecules including miRNAs, the origin and functional significance of which are still undetermined. We used TaqMan OpenArrays to profile 681 mature miRNAs in HM cells and fat, and compared them with maternal peripheral blood mononuclear cells (PBMCs) and plasma...

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Bibliographic Details
Published in:Scientific reports 2016-02, Vol.6 (1), p.20680-20680, Article 20680
Main Authors: Alsaweed, Mohammed, Lai, Ching Tat, Hartmann, Peter E., Geddes, Donna T., Kakulas, Foteini
Format: Article
Language:English
Subjects:
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Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
Baby foods
Breast milk
Cattle
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial-Mesenchymal Transition
Epithelium
Female
Food
Humanities and Social Sciences
Humans
Infant Formula - analysis
Infant, Newborn
Infants
Lactation
Leukocytes (mononuclear)
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Mammary gland
Mammary Glands, Human - cytology
Mammary Glands, Human - metabolism
MicroRNAs - blood
MicroRNAs - metabolism
Milk
Milk, Human - metabolism
miRNA
multidisciplinary
Oligonucleotide Array Sequence Analysis
Peripheral blood mononuclear cells
Principal Component Analysis
Real-Time Polymerase Chain Reaction
Science
Science (multidisciplinary)
Transcriptome
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Summary:Human milk (HM) contains regulatory biomolecules including miRNAs, the origin and functional significance of which are still undetermined. We used TaqMan OpenArrays to profile 681 mature miRNAs in HM cells and fat, and compared them with maternal peripheral blood mononuclear cells (PBMCs) and plasma, and bovine and soy infant formulae. HM cells and PBMCs (292 and 345 miRNAs, respectively) had higher miRNA content than HM fat and plasma (242 and 219 miRNAs, respectively) ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/srep20680