Multi-tissue transcriptomics for construction of a comprehensive gene resource for the terrestrial snail Theba pisana

The land snail Theba pisana is native to the Mediterranean region but has become one of the most abundant invasive species worldwide. Here, we present three transcriptomes of this agriculture pest derived from three tissues: the central nervous system, hepatopancreas (digestive gland), and foot musc...

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Bibliographic Details
Published in:Scientific reports 2016-02, Vol.6 (1), p.20685-20685, Article 20685
Main Authors: Zhao, M., Wang, T., Adamson, K. J., Storey, K. B., Cummins, S. F.
Format: Article
Language:English
Subjects:
45/91
631/114/129/2044
631/158/670
Animals
Bacterial diseases
Browsing
Central nervous system
Central Nervous System - metabolism
Contig Mapping
Databases, Genetic
Digestive glands
Feet
Foot
Gene mapping
Genes
Genetic resources
Hepatopancreas
Hepatopancreas - metabolism
Humanities and Social Sciences
Internet
Introduced species
Invasive species
multidisciplinary
Muscle, Skeletal - metabolism
Neurological diseases
Science
Snails - genetics
Transcriptome
User-Computer Interface
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Summary:The land snail Theba pisana is native to the Mediterranean region but has become one of the most abundant invasive species worldwide. Here, we present three transcriptomes of this agriculture pest derived from three tissues: the central nervous system, hepatopancreas (digestive gland), and foot muscle. Sequencing of the three tissues produced 339,479,092 high quality reads and a global de novo assembly generated a total of 250,848 unique transcripts (unigenes). BLAST analysis mapped 52,590 unigenes to NCBI non-redundant protein databases and further functional analysis annotated 21,849 unigenes with gene ontology. We report that T. pisana transcripts have representatives in all functional classes and a comparison of differentially expressed transcripts amongst all three tissues demonstrates enormous differences in their potential metabolic activities. The genes differentially expressed include those with sequence similarity to those genes associated with multiple bacterial diseases and neurological diseases. To provide a valuable resource that will assist functional genomics study, we have implemented a user-friendly web interface, ThebaDB ( http://thebadb.bioinfo-minzhao.org/ ). This online database allows for complex text queries, sequence searches, and data browsing by enriched functional terms and KEGG mapping.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep20685