NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1

• Published in: Cell research 2016-02, Vol.26 (2), p.239-253
• Main Authors: Zhang, Cheng, Zhang, Wen, Lu, Yi, Yan, Xiaoyi, Yan, Xiumin, Zhu, Xueliang, Liu, Wei, Yang, Yuehong, Zhou, Tianhua
• Format: Article
• Language: English
• Subjects: 631/136/2432; 631/80/128/1276; 631/80/86; Actins - metabolism; Animals; Biomedical and Life Sciences; Cell Biology; Cells, Cultured; Cilia - metabolism; Cilia - physiology; Cofilin 1 - metabolism; Edema; Hsp90; HSP90 Heat-Shock Proteins; Life Sciences; Morphogenesis - physiology; Nuclear Proteins - metabolism; Original; original-article; Zebrafish - metabolism; Zebrafish - physiology; 培养细胞; 左右不对称; 异位表达; 稳定; 纤毛细胞; 组织动力学; 肌动蛋白细胞骨架
• ISSN: 1001-0602; 1748-7838
• DOI: 10.1038/cr.2015.152

Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C （NudC）, an Hsp90 co-chaperone, is required for ac- tin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates aetin cytoskeleton and ciliogenesis by stabilizing cofilin 1.