NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1

Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C （NudC）, an Hsp90 co-chaperone, is required for ac- tin organization and dynamics. Depletion of NudC promotes cilia elongation a...

Full description

Saved in:
Bibliographic Details
Published in:Cell research 2016-02, Vol.26 (2), p.239-253
Main Authors: Zhang, Cheng, Zhang, Wen, Lu, Yi, Yan, Xiaoyi, Yan, Xiumin, Zhu, Xueliang, Liu, Wei, Yang, Yuehong, Zhou, Tianhua
Format: Article
Language:English
Subjects:
631/136/2432
631/80/128/1276
631/80/86
Actins - metabolism
Animals
Biomedical and Life Sciences
Cell Biology
Cells, Cultured
Cilia - metabolism
Cilia - physiology
Cofilin 1 - metabolism
Edema
Hsp90
HSP90 Heat-Shock Proteins
Life Sciences
Morphogenesis - physiology
Nuclear Proteins - metabolism
Original
original-article
Zebrafish - metabolism
Zebrafish - physiology
培养细胞
左右不对称
异位表达
稳定
纤毛细胞
组织动力学
肌动蛋白细胞骨架
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3
cites cdi_FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3
container_end_page 253
container_issue 2
container_start_page 239
container_title Cell research
container_volume 26
creator Zhang, Cheng
Zhang, Wen
Lu, Yi
Yan, Xiaoyi
Yan, Xiumin
Zhu, Xueliang
Liu, Wei
Yang, Yuehong
Zhou, Tianhua
description Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C （NudC）, an Hsp90 co-chaperone, is required for ac- tin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates aetin cytoskeleton and ciliogenesis by stabilizing cofilin 1.
doi_str_mv 10.1038/cr.2015.152
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4746604</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>668902289</cqvip_id><sourcerecordid>3943545061</sourcerecordid><originalsourceid>FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3</originalsourceid><addsrcrecordid>eNptkU1v1DAQhq2qqC1LT72jqFwqQZbxZ5ILElpBQaraC5wtx5mkrrJ2ayeVll9fl11WBXHyeOaZd2b0EnJGYUmB1x9tXDKgckklOyAntBJ1WdW8PswxAC1BATsmr1O6A2BSSHpEjpmqQOTwhFxez92qiDjMo5kwFcZOzhfdxpu1s_nru8K60YUBPSaXinZTpMm0OfXL-aGwoc-hL-gb8qo3Y8LT3bsgP79--bH6Vl7dXH5ffb4qrRR8KntlOivBtLyxTEKPVd30iB3tpeSGCsYgnwSqw9pwVG3FKTMqN6mOKyNbviCftrr3c7vGzqKfohn1fXRrEzc6GKf_rnh3q4fwqEUllAKRBS52AjE8zJgmvXbJ4jgaj2FOmlaKNbIRSmb03T_oXZijz-f9plQlIRuwIO-3lI0hpYj9fhkK-tkgbaN-NkhngzL99uX-e_aPIxn4sAVSLvkB44uh_9U7302_DX54yB17SaXqBhirG_4ECsalew</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1762675010</pqid></control><display><type>article</type><title>NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1</title><source>NCBI_PubMed Central（免费）</source><creator>Zhang, Cheng ; Zhang, Wen ; Lu, Yi ; Yan, Xiaoyi ; Yan, Xiumin ; Zhu, Xueliang ; Liu, Wei ; Yang, Yuehong ; Zhou, Tianhua</creator><creatorcontrib>Zhang, Cheng ; Zhang, Wen ; Lu, Yi ; Yan, Xiaoyi ; Yan, Xiumin ; Zhu, Xueliang ; Liu, Wei ; Yang, Yuehong ; Zhou, Tianhua</creatorcontrib><description>Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C （NudC）, an Hsp90 co-chaperone, is required for ac- tin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates aetin cytoskeleton and ciliogenesis by stabilizing cofilin 1.</description><identifier>ISSN: 1001-0602</identifier><identifier>EISSN: 1748-7838</identifier><identifier>DOI: 10.1038/cr.2015.152</identifier><identifier>PMID: 26704451</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/136/2432 ; 631/80/128/1276 ; 631/80/86 ; Actins - metabolism ; Animals ; Biomedical and Life Sciences ; Cell Biology ; Cells, Cultured ; Cilia - metabolism ; Cilia - physiology ; Cofilin 1 - metabolism ; Edema ; Hsp90 ; HSP90 Heat-Shock Proteins ; Life Sciences ; Morphogenesis - physiology ; Nuclear Proteins - metabolism ; Original ; original-article ; Zebrafish - metabolism ; Zebrafish - physiology ; 培养细胞 ; 左右不对称 ; 异位表达 ; 稳定 ; 纤毛细胞 ; 组织动力学 ; 肌动蛋白细胞骨架</subject><ispartof>Cell research, 2016-02, Vol.26 (2), p.239-253</ispartof><rights>Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2016</rights><rights>Copyright Nature Publishing Group Feb 2016</rights><rights>Copyright © 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3</citedby><cites>FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85240X/85240X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746604/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746604/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26704451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Cheng</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Yan, Xiaoyi</creatorcontrib><creatorcontrib>Yan, Xiumin</creatorcontrib><creatorcontrib>Zhu, Xueliang</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Yang, Yuehong</creatorcontrib><creatorcontrib>Zhou, Tianhua</creatorcontrib><title>NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1</title><title>Cell research</title><addtitle>Cell Res</addtitle><addtitle>Cell Research</addtitle><description>Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C （NudC）, an Hsp90 co-chaperone, is required for ac- tin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates aetin cytoskeleton and ciliogenesis by stabilizing cofilin 1.</description><subject>631/136/2432</subject><subject>631/80/128/1276</subject><subject>631/80/86</subject><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cells, Cultured</subject><subject>Cilia - metabolism</subject><subject>Cilia - physiology</subject><subject>Cofilin 1 - metabolism</subject><subject>Edema</subject><subject>Hsp90</subject><subject>HSP90 Heat-Shock Proteins</subject><subject>Life Sciences</subject><subject>Morphogenesis - physiology</subject><subject>Nuclear Proteins - metabolism</subject><subject>Original</subject><subject>original-article</subject><subject>Zebrafish - metabolism</subject><subject>Zebrafish - physiology</subject><subject>培养细胞</subject><subject>左右不对称</subject><subject>异位表达</subject><subject>稳定</subject><subject>纤毛细胞</subject><subject>组织动力学</subject><subject>肌动蛋白细胞骨架</subject><issn>1001-0602</issn><issn>1748-7838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNptkU1v1DAQhq2qqC1LT72jqFwqQZbxZ5ILElpBQaraC5wtx5mkrrJ2ayeVll9fl11WBXHyeOaZd2b0EnJGYUmB1x9tXDKgckklOyAntBJ1WdW8PswxAC1BATsmr1O6A2BSSHpEjpmqQOTwhFxez92qiDjMo5kwFcZOzhfdxpu1s_nru8K60YUBPSaXinZTpMm0OfXL-aGwoc-hL-gb8qo3Y8LT3bsgP79--bH6Vl7dXH5ffb4qrRR8KntlOivBtLyxTEKPVd30iB3tpeSGCsYgnwSqw9pwVG3FKTMqN6mOKyNbviCftrr3c7vGzqKfohn1fXRrEzc6GKf_rnh3q4fwqEUllAKRBS52AjE8zJgmvXbJ4jgaj2FOmlaKNbIRSmb03T_oXZijz-f9plQlIRuwIO-3lI0hpYj9fhkK-tkgbaN-NkhngzL99uX-e_aPIxn4sAVSLvkB44uh_9U7302_DX54yB17SaXqBhirG_4ECsalew</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Zhang, Cheng</creator><creator>Zhang, Wen</creator><creator>Lu, Yi</creator><creator>Yan, Xiaoyi</creator><creator>Yan, Xiumin</creator><creator>Zhu, Xueliang</creator><creator>Liu, Wei</creator><creator>Yang, Yuehong</creator><creator>Zhou, Tianhua</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160201</creationdate><title>NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1</title><author>Zhang, Cheng ; Zhang, Wen ; Lu, Yi ; Yan, Xiaoyi ; Yan, Xiumin ; Zhu, Xueliang ; Liu, Wei ; Yang, Yuehong ; Zhou, Tianhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/136/2432</topic><topic>631/80/128/1276</topic><topic>631/80/86</topic><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cells, Cultured</topic><topic>Cilia - metabolism</topic><topic>Cilia - physiology</topic><topic>Cofilin 1 - metabolism</topic><topic>Edema</topic><topic>Hsp90</topic><topic>HSP90 Heat-Shock Proteins</topic><topic>Life Sciences</topic><topic>Morphogenesis - physiology</topic><topic>Nuclear Proteins - metabolism</topic><topic>Original</topic><topic>original-article</topic><topic>Zebrafish - metabolism</topic><topic>Zebrafish - physiology</topic><topic>培养细胞</topic><topic>左右不对称</topic><topic>异位表达</topic><topic>稳定</topic><topic>纤毛细胞</topic><topic>组织动力学</topic><topic>肌动蛋白细胞骨架</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Cheng</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Yan, Xiaoyi</creatorcontrib><creatorcontrib>Yan, Xiumin</creatorcontrib><creatorcontrib>Zhu, Xueliang</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Yang, Yuehong</creatorcontrib><creatorcontrib>Zhou, Tianhua</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Cheng</au><au>Zhang, Wen</au><au>Lu, Yi</au><au>Yan, Xiaoyi</au><au>Yan, Xiumin</au><au>Zhu, Xueliang</au><au>Liu, Wei</au><au>Yang, Yuehong</au><au>Zhou, Tianhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1</atitle><jtitle>Cell research</jtitle><stitle>Cell Res</stitle><addtitle>Cell Research</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>26</volume><issue>2</issue><spage>239</spage><epage>253</epage><pages>239-253</pages><issn>1001-0602</issn><eissn>1748-7838</eissn><abstract>Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C （NudC）, an Hsp90 co-chaperone, is required for ac- tin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates aetin cytoskeleton and ciliogenesis by stabilizing cofilin 1.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26704451</pmid><doi>10.1038/cr.2015.152</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1001-0602
ispartof Cell research, 2016-02, Vol.26 (2), p.239-253
issn 1001-0602
1748-7838
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4746604
source NCBI_PubMed Central（免费）
subjects 631/136/2432
631/80/128/1276
631/80/86
Actins - metabolism
Animals
Biomedical and Life Sciences
Cell Biology
Cells, Cultured
Cilia - metabolism
Cilia - physiology
Cofilin 1 - metabolism
Edema
Hsp90
HSP90 Heat-Shock Proteins
Life Sciences
Morphogenesis - physiology
Nuclear Proteins - metabolism
Original
original-article
Zebrafish - metabolism
Zebrafish - physiology
培养细胞
左右不对称
异位表达
稳定
纤毛细胞
组织动力学
肌动蛋白细胞骨架
title NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T11%3A18%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=NudC%20regulates%20actin%20dynamics%20and%20ciliogenesis%20by%20stabilizing%20cofilin%201&rft.jtitle=Cell%20research&rft.au=Zhang,%20Cheng&rft.date=2016-02-01&rft.volume=26&rft.issue=2&rft.spage=239&rft.epage=253&rft.pages=239-253&rft.issn=1001-0602&rft.eissn=1748-7838&rft_id=info:doi/10.1038/cr.2015.152&rft_dat=%3Cproquest_pubme%3E3943545061%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c543t-f6adc50ab39c250fe789feed1f553a1422003806de8a3e6b7312a66ad6d36a5b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1762675010&rft_id=info:pmid/26704451&rft_cqvip_id=668902289&rfr_iscdi=true