Kinetics of functionalised carbon nanotube distribution in mouse brain after systemic injection: Spatial to ultra-structural analyses

• Published in: Journal of controlled release 2016-02, Vol.224, p.22-32
• Main Authors: Wang, Julie T.-W., Rubio, Noelia, Kafa, Houmam, Venturelli, Enrica, Fabbro, Chiara, Ménard-Moyon, Cécilia, Da Ros, Tatiana, Sosabowski, Jane K., Lawson, Alastair D., Robinson, Martyn K., Prato, Maurizio, Bianco, Alberto, Festy, Frederic, Preston, Jane E., Kostarelos, Kostas, Al-Jamal, Khuloud T.
• Format: Article
• Language: English
• Subjects: Animals; Autoradiography; Blood-Brain Barrier - metabolism; Blood–brain barrier; Brain - diagnostic imaging; Brain - drug effects; Brain - metabolism; Brain drug delivery; Capillaries - metabolism; Carbon nanotubes; Chemical Sciences; Dexamethasone - pharmacology; Drug Carriers; Endothelium - metabolism; Female; Medicinal Chemistry; Mice; Mice, Inbred C57BL; Microscopy, Electron, Transmission; Multi-photon luminescence microscopy; Nanomedicine; Nanotubes, Carbon; SPECT/CT imaging; Spectrum Analysis, Raman; Tissue Distribution; Tomography, Emission-Computed, Single-Photon
• ISSN: 0168-3659; 1873-4995; 1873-4995
• DOI: 10.1016/j.jconrel.2015.12.039

Earlier studies proved the success of using chemically functionalised multi-walled carbon nanotubes (f-MWNTs) as nanocarriers to the brain. Little insight into the kinetics of brain distribution of f-MWNTs in vivo has been reported. This study employed a wide range of qualitative and quantitative techniques with the aim of shedding the light on f-MWNT's brain distribution following intravenous injection. γ-Scintigraphy quantified the uptake of studied radiolabelled f-MWNT in the whole brain parenchyma and capillaries while 3D-single photon emission computed tomography/computed tomography imaging and autoradiography illustrated spatial distribution within various brain regions. Raman and multiphoton luminescence together with transmission electron microscopy confirmed the presence of intact f-MWNT in mouse brain, in a label-free manner. The results evidenced the presence of f-MWNT in mice brain parenchyma, in addition to brain endothelium. Such information on the rate and extent of regional and cellular brain distribution is needed before further implementation into neurological therapeutics can be made. [Display omitted]