Immunosuppression by pulmonary surfactant: mechanisms of action

Pulmonary surfactant has been shown by this group to suppress peripheral blood lymphocyte responses to mitogens and alloantigens in a dose dependent manner, though the mechanism of action of the suppressive effect is not clearly understood. To try to clarify this, attempts were made to reverse the e...

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Bibliographic Details
Published in:Thorax 1990-01, Vol.45 (1), p.3-8
Main Authors: Wilsher, M L, Parker, D J, Haslam, P L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cells, Cultured
Humans
Immune Tolerance - immunology
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Indomethacin - pharmacology
Lymphocytes - drug effects
Lymphocytes - immunology
Lymphocytes - metabolism
Medical sciences
Monocytes - immunology
Phytohemagglutinins - pharmacology
Pulmonary Surfactants - immunology
Swine
Thymidine - metabolism
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Summary:Pulmonary surfactant has been shown by this group to suppress peripheral blood lymphocyte responses to mitogens and alloantigens in a dose dependent manner, though the mechanism of action of the suppressive effect is not clearly understood. To try to clarify this, attempts were made to reverse the effects of preincubation with surfactant, obtained by bronchoalveolar lavage from pigs, on lymphocytes and accessory monocytes obtained from the blood of normal volunteers, by washing and incubating the cells in medium alone for various periods up to 24 hours. Immunosuppression, measured as the reduction in thymidine incorporation in response to the mitogen phytohaemagglutinin, could not be reversed by these methods. The addition of indomethacin (up to 100 micrograms/ml for 72 hours) also had no effect, indicating that the immunosuppression was not related to synthesis of prostaglandins. Incubation with surfactant for as little as two hours before addition of mitogen suppressed in vitro lymphoproliferative responses by half, but surfactant added two hours after mitogen had no observed effect. Preincubation of purified lymphocytes in surfactant, before they were cultured with accessory monocytes and mitogen, caused significant suppression of response, but preincubation of purified monocytes had no suppressive effect. There was no change in the intensity of HLA-DR expression on monocytes. These results support the hypothesis that surfactant exerts its effects on the resting uncommitted lymphocyte rather than on antigen presenting monocytes.
ISSN:0040-6376
1468-3296
DOI:10.1136/thx.45.1.3