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Nanoliposomes protect against human arteriole endothelial dysfunction induced by β-amyloid peptide

We tested whether nanoliposomes containing phosphatidylcholine, cholesterol and phosphatidic acid (NLPA) prevent β-amyloid 1-42 (Aβ42) fibrillation and Aβ42-induced human arteriole endothelial dysfunction. NLPA abolished Aβ42 fibril formation (thioflavin-T fluorescence/electron microscopy). In ex-vi...

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Bibliographic Details
Published in:Journal of cerebral blood flow and metabolism 2016-02, Vol.36 (2), p.405-412
Main Authors: Truran, Seth, Weissig, Volkmar, Madine, Jillian, Davies, Hannah A, Guzman-Villanueva, Diana, Franco, Daniel A, Karamanova, Nina, Burciu, Camelia, Serrano, Geidy, Beach, Thomas G, Migrino, Raymond Q
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Language:English
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Summary:We tested whether nanoliposomes containing phosphatidylcholine, cholesterol and phosphatidic acid (NLPA) prevent β-amyloid 1-42 (Aβ42) fibrillation and Aβ42-induced human arteriole endothelial dysfunction. NLPA abolished Aβ42 fibril formation (thioflavin-T fluorescence/electron microscopy). In ex-vivo human adipose and leptomeningeal arterioles, Aβ42 impaired dilator response to acetylcholine that was reversed by NLPA; this protection was abolished by L-NG-nitroarginine methyl ester. Aβ42 reduced human umbilical vein endothelial cell NO production that was restored by NLPA. Nanoliposomes prevented Aβ42 amyloid formation, reversed Aβ42-induced human microvascular endothelial dysfunction and may be useful in Alzheimer’s disease.
ISSN:0271-678X
1559-7016
DOI:10.1177/0271678X15610134