Surface Molecularly Imprinted Polymer of Chitosan Grafted Poly(methyl methacrylate) for 5-Fluorouracil and Controlled Release

• Published in: Scientific reports 2016-02, Vol.6 (1), p.21409-21409, Article 21409
• Main Authors: Zheng, Xue-Fang, Lian, Qi, Yang, Hua, Wang, Xiuping
• Format: Article
• Language: English
• Subjects: 5-Fluorouracil; 631/1647/350/354; 631/337; 631/45; Adsorption; Ammonium; Binding sites; Cancer therapies; Chemical engineering; Chemical reactions; Chemotherapy; Chitosan; Chitosan - chemistry; Colon; Colorectal cancer; Controlled release; Delayed-Action Preparations; Drug Carriers - chemistry; Drug delivery; Drug Delivery Systems; Drug Liberation; Fluorouracil - administration & dosage; Gravimetric analysis; Humanities and Social Sciences; Hydrogen bonds; Hydrogen-Ion Concentration; Immunosuppressive agents; Intestine; Microspheres; Molecular Imprinting - methods; multidisciplinary; pH effects; Polymerization; Polymers; Polymethyl Methacrylate - chemistry; Polymethylmethacrylate; Proton Magnetic Resonance Spectroscopy; Science; Small intestine; Spectroscopy, Fourier Transform Infrared; Temperature; Temperature effects; Thermogravimetry; X-Ray Diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep21409

The molecular surface imprinted graft copolymer of chitosan with methyl methacrylate (MIP-CS-g-PMMA) were prepared by free radical polymerization with 5-fluorouracil (5-FU) as the template molecule using initiator of ammonium persulfate as adsorption system. MIPs were characterized by FTIR, X-ray diffraction, thermo-gravimetric analysis, 1 H NMR and SEM. The mechanism of graft copolymerization and factors affected graft reaction were studied in details and the optimum reaction conditions (to the highest %G and %E as the standard) were obtained at [MMA] 1.2 mol/L, [Chitosan] 16.67 mol/L, [initiator] 0.0062 mol/L, temperature 60 °C and reaction time 7 h. MIPs exhibited high recognition selectivity and excellent combining affinity to template molecular. The in vitro release of the 5-FU was highly pH-dependent and time delayed. The release behavior showed that the drugs did not release in simulated gastric fluid (pH = 1.0) and the drug release was small in the simulated small intestinal fluid (pH = 6.8) and drug abrupt release will be produced in the simulated colon fluid (pH = 7.4), indicating excellent colon-specific drug delivery behavior.