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Computer Aided–Designed, 3-Dimensionally Printed Porous Tissue Bioscaffolds for Craniofacial Soft Tissue Reconstruction

Objective To determine the potential of an integrated, image-based computer-aided design (CAD) and 3-dimensional (3D) printing approach to engineer scaffolds for head and neck cartilaginous reconstruction for auricular and nasal reconstruction. Study Design Proof of concept revealing novel methods f...

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Published in:Otolaryngology-head and neck surgery 2015-01, Vol.152 (1), p.57-62
Main Authors: Zopf, David A., Mitsak, Anna G., Flanagan, Colleen L., Wheeler, Matthew, Green, Glenn E., Hollister, Scott J.
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cited_by cdi_FETCH-LOGICAL-c5498-2f8aab1d9a8b68975186fa46ade1e938f73e3ea586d8a17dbfe3d972f0c676673
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container_start_page 57
container_title Otolaryngology-head and neck surgery
container_volume 152
creator Zopf, David A.
Mitsak, Anna G.
Flanagan, Colleen L.
Wheeler, Matthew
Green, Glenn E.
Hollister, Scott J.
description Objective To determine the potential of an integrated, image-based computer-aided design (CAD) and 3-dimensional (3D) printing approach to engineer scaffolds for head and neck cartilaginous reconstruction for auricular and nasal reconstruction. Study Design Proof of concept revealing novel methods for bioscaffold production with in vitro and in vivo animal data. Setting Multidisciplinary effort encompassing 2 academic institutions. Subjects and Methods Digital Imaging and Communications in Medicine (DICOM) computed tomography scans were segmented and utilized in image-based CAD to create porous, anatomic structures. Bioresorbable polycaprolactone scaffolds with spherical and random porous architecture were produced using a laser-based 3D printing process. Subcutaneous in vivo implantation of auricular and nasal scaffolds was performed in a porcine model. Auricular scaffolds were seeded with chondrogenic growth factors in a hyaluronic acid/collagen hydrogel and cultured in vitro over 2 months’ duration. Results Auricular and nasal constructs with several types of microporous architecture were rapidly manufactured with high fidelity to human patient anatomy. Subcutaneous in vivo implantation of auricular and nasal scaffolds resulted in an excellent appearance and complete soft tissue ingrowth. Histological analysis of in vitro scaffolds demonstrated native-appearing cartilaginous growth that respected the boundaries of the scaffold. Conclusion Integrated, image-based CAD and 3D printing processes generated patient-specific nasal and auricular scaffolds that supported cartilage regeneration.
doi_str_mv 10.1177/0194599814552065
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Study Design Proof of concept revealing novel methods for bioscaffold production with in vitro and in vivo animal data. Setting Multidisciplinary effort encompassing 2 academic institutions. Subjects and Methods Digital Imaging and Communications in Medicine (DICOM) computed tomography scans were segmented and utilized in image-based CAD to create porous, anatomic structures. Bioresorbable polycaprolactone scaffolds with spherical and random porous architecture were produced using a laser-based 3D printing process. Subcutaneous in vivo implantation of auricular and nasal scaffolds was performed in a porcine model. Auricular scaffolds were seeded with chondrogenic growth factors in a hyaluronic acid/collagen hydrogel and cultured in vitro over 2 months’ duration. Results Auricular and nasal constructs with several types of microporous architecture were rapidly manufactured with high fidelity to human patient anatomy. Subcutaneous in vivo implantation of auricular and nasal scaffolds resulted in an excellent appearance and complete soft tissue ingrowth. Histological analysis of in vitro scaffolds demonstrated native-appearing cartilaginous growth that respected the boundaries of the scaffold. Conclusion Integrated, image-based CAD and 3D printing processes generated patient-specific nasal and auricular scaffolds that supported cartilage regeneration.</description><identifier>ISSN: 0194-5998</identifier><identifier>EISSN: 1097-6817</identifier><identifier>DOI: 10.1177/0194599814552065</identifier><identifier>PMID: 25281749</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>3‐dimensional printing ; Animals ; anotia ; auricular reconstruction ; Bioprosthesis ; CAD/CAM ; Computer-Aided Design ; computer‐aided manufacturing ; craniofacial reconstruction ; Ear - surgery ; Face ; Humans ; microtia ; nasal reconstruction ; Nose - surgery ; Plastic Surgery Procedures - methods ; Printing, Three-Dimensional ; Skull ; Swine ; tissue engineering ; Tissue Scaffolds</subject><ispartof>Otolaryngology-head and neck surgery, 2015-01, Vol.152 (1), p.57-62</ispartof><rights>American Academy of Otolaryngology—Head and Neck Surgery Foundation 2014</rights><rights>2015 American Association of Otolaryngology‐Head and Neck Surgery Foundation (AAO‐HNSF)</rights><rights>American Academy of Otolaryngology—Head and Neck Surgery Foundation 2014.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5498-2f8aab1d9a8b68975186fa46ade1e938f73e3ea586d8a17dbfe3d972f0c676673</citedby><cites>FETCH-LOGICAL-c5498-2f8aab1d9a8b68975186fa46ade1e938f73e3ea586d8a17dbfe3d972f0c676673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25281749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zopf, David A.</creatorcontrib><creatorcontrib>Mitsak, Anna G.</creatorcontrib><creatorcontrib>Flanagan, Colleen L.</creatorcontrib><creatorcontrib>Wheeler, Matthew</creatorcontrib><creatorcontrib>Green, Glenn E.</creatorcontrib><creatorcontrib>Hollister, Scott J.</creatorcontrib><title>Computer Aided–Designed, 3-Dimensionally Printed Porous Tissue Bioscaffolds for Craniofacial Soft Tissue Reconstruction</title><title>Otolaryngology-head and neck surgery</title><addtitle>Otolaryngol Head Neck Surg</addtitle><description>Objective To determine the potential of an integrated, image-based computer-aided design (CAD) and 3-dimensional (3D) printing approach to engineer scaffolds for head and neck cartilaginous reconstruction for auricular and nasal reconstruction. Study Design Proof of concept revealing novel methods for bioscaffold production with in vitro and in vivo animal data. Setting Multidisciplinary effort encompassing 2 academic institutions. Subjects and Methods Digital Imaging and Communications in Medicine (DICOM) computed tomography scans were segmented and utilized in image-based CAD to create porous, anatomic structures. Bioresorbable polycaprolactone scaffolds with spherical and random porous architecture were produced using a laser-based 3D printing process. Subcutaneous in vivo implantation of auricular and nasal scaffolds was performed in a porcine model. Auricular scaffolds were seeded with chondrogenic growth factors in a hyaluronic acid/collagen hydrogel and cultured in vitro over 2 months’ duration. Results Auricular and nasal constructs with several types of microporous architecture were rapidly manufactured with high fidelity to human patient anatomy. Subcutaneous in vivo implantation of auricular and nasal scaffolds resulted in an excellent appearance and complete soft tissue ingrowth. Histological analysis of in vitro scaffolds demonstrated native-appearing cartilaginous growth that respected the boundaries of the scaffold. Conclusion Integrated, image-based CAD and 3D printing processes generated patient-specific nasal and auricular scaffolds that supported cartilage regeneration.</description><subject>3‐dimensional printing</subject><subject>Animals</subject><subject>anotia</subject><subject>auricular reconstruction</subject><subject>Bioprosthesis</subject><subject>CAD/CAM</subject><subject>Computer-Aided Design</subject><subject>computer‐aided manufacturing</subject><subject>craniofacial reconstruction</subject><subject>Ear - surgery</subject><subject>Face</subject><subject>Humans</subject><subject>microtia</subject><subject>nasal reconstruction</subject><subject>Nose - surgery</subject><subject>Plastic Surgery Procedures - methods</subject><subject>Printing, Three-Dimensional</subject><subject>Skull</subject><subject>Swine</subject><subject>tissue engineering</subject><subject>Tissue Scaffolds</subject><issn>0194-5998</issn><issn>1097-6817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkb9uFDEYxC0EIkegp0IuKViwd9f_GqTkQghSRCIIteWzPx-Odu3D3gVdxzvwhjwJPl0SARKicjG_GY89CD2l5CWlQrwiVPVMKUl7xlrC2T20oESJhksq7qPFTm52-gF6VMo1IYRzIR6ig5a1lejVAm2XadzME2R8FBy4n99_nEAJ6wjuBe6akzBCLCFFMwxbfJlDnMDhy5TTXPBVKGUGfBxSscb7NLiCfcp4mU0MyRsbzIA_Jj_dkh_AplimPNupRj5GD7wZCjy5OQ_Rp9M3V8uz5vzi7bvl0XljWa9k03ppzIo6ZeSKSyUYldybnhsHFFQnveigA8Mkd9JQ4VYeOqdE64nloj63O0Sv97mbeTWCsxCnbAa9yWE0eauTCfpPJYbPep2-6l5wIpmsAc9vAnL6MkOZ9BiKhWEwEeo_aMp72hLKRFtRskdtTqVk8HfXUKJ3i-m_F6uWZ7_XuzPcTlQBuQe-hQG2_w3UF2fvj09roW7XvNlbi1mDvk5zrkOWf3f5BXA3sio</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Zopf, David A.</creator><creator>Mitsak, Anna G.</creator><creator>Flanagan, Colleen L.</creator><creator>Wheeler, Matthew</creator><creator>Green, Glenn E.</creator><creator>Hollister, Scott J.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201501</creationdate><title>Computer Aided–Designed, 3-Dimensionally Printed Porous Tissue Bioscaffolds for Craniofacial Soft Tissue Reconstruction</title><author>Zopf, David A. ; 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source Wiley-Blackwell Read & Publish Collection
subjects 3‐dimensional printing
Animals
anotia
auricular reconstruction
Bioprosthesis
CAD/CAM
Computer-Aided Design
computer‐aided manufacturing
craniofacial reconstruction
Ear - surgery
Face
Humans
microtia
nasal reconstruction
Nose - surgery
Plastic Surgery Procedures - methods
Printing, Three-Dimensional
Skull
Swine
tissue engineering
Tissue Scaffolds
title Computer Aided–Designed, 3-Dimensionally Printed Porous Tissue Bioscaffolds for Craniofacial Soft Tissue Reconstruction
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